Literature summary for 3.1.21.1 extracted from

Fukano, H.; Suzuki, Y.
Enzymatic conversion of long DNA to small DNA fragments for the construction of short hairpin RNA expression libraries (2009), Anal. Biochem., 385, 80-84.

Search for more literature for 3.1.21.1

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	DNA fragmentation by digestion with DNase I in the presence of Mg2+ followed by blunting with the Klenow fragment improves the yield of small DNA fragments by 4fold as compared to the reaction with DNase I in the presence of Mn2+. In the case of using Mg2+ as a cofactor, DNase I attacks each strand of dsDNA independently	Homo sapiens
Mn2+	DNA fragmentation by digestion with DNase I in the presence of Mn2+ followed by blunting with T4 DNA polymerase is 4fold less effective than the reaction with DNase I in the presence of Mg2+. In the reaction with DNase I in the presence of Mn2+, one molecule of DNA is completely cleaved to produce two molecules of DNA	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
double-stranded DNA + H2O	-	Homo sapiens	5'-phosphooligonucleotides + ?	-	?

Synonyms

Synonyms	Comment	Organism
deoxyribonuclease I	-	Homo sapiens
DNase I	-	Homo sapiens

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Release 2023.1 (January 2023)