Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.21.1 extracted from

  • Chen, W.J.; Lo, T.; Lai, Y.S.; Huang, P.T.; Lin, C.C.; Liao, T.H.
    Construction and characterization of a bifunctional enzyme with deoxyribonuclease I and thioredoxin-like activities (2007), Biochem. Biophys. Res. Commun., 356, 750-755.
    View publication on PubMed
Search for more literature for 3.1.21.1

Protein Variants

Protein Variants Comment Organism
E102G/S103P mutation in non-essential disulfide motif CESC that exhibits thioredoxn-like activity. DNase I activity similar to wild-type, 4fold increase in thioredoxin-like activity Bos taurus
E102P/S103G mutation in non-essential disulfide motif CESC that exhibits thioredoxn-like activity. DNase I activity similar to wild-type, 4fold increase in thioredoxin-like activity Bos taurus
G100K/E102P/S103G/G105W mutation in non-essential disulfide motif CESC that exhibits thioredoxn-like activity. DNase I activity about half of wild-type, 4.5fold increase in thioredoxin-like activity Bos taurus
G100K/G105W mutation in non-essential disulfide motif CESC that exhibits thioredoxn-like activity. DNase I and thioredoxin-like activity similar to wild-type Bos taurus
G100W/E102/G/S103P/G105K mutation in non-essential disulfide motif CESC that exhibits thioredoxn-like activity. DNase I activity similar to wild-type, 6fold increase in thioredoxin-like activity Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus P00639
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information protein exhibits one large essential (C173-C209) disulfide loop and one small non-essential (C101-C104) disulfide loop Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
531
-
 mutant G100K/E102P/S103G/G105W, pH 7.0 Bos taurus
962
-
 mutant E102P/S103G, pH 7.0 Bos taurus
968
-
 mutant G100K/G105W, pH 7.0 Bos taurus
1009
-
 wild-type, pH 7.0 Bos taurus
1020
-
 mutant G100W/E102/G/S103P/G105K, pH 7.0 Bos taurus
1092
-
 mutant E102G/S103P, pH 7.0 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
calf thymus DNA + H2O
-
 Bos taurus 5'-phosphooligonucleotides + ?
-
 ?