Protein Variants | Comment | Organism |
---|---|---|
E102G/S103P | mutation in non-essential disulfide motif CESC that exhibits thioredoxn-like activity. DNase I activity similar to wild-type, 4fold increase in thioredoxin-like activity | Bos taurus |
E102P/S103G | mutation in non-essential disulfide motif CESC that exhibits thioredoxn-like activity. DNase I activity similar to wild-type, 4fold increase in thioredoxin-like activity | Bos taurus |
G100K/E102P/S103G/G105W | mutation in non-essential disulfide motif CESC that exhibits thioredoxn-like activity. DNase I activity about half of wild-type, 4.5fold increase in thioredoxin-like activity | Bos taurus |
G100K/G105W | mutation in non-essential disulfide motif CESC that exhibits thioredoxn-like activity. DNase I and thioredoxin-like activity similar to wild-type | Bos taurus |
G100W/E102/G/S103P/G105K | mutation in non-essential disulfide motif CESC that exhibits thioredoxn-like activity. DNase I activity similar to wild-type, 6fold increase in thioredoxin-like activity | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P00639 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | protein exhibits one large essential (C173-C209) disulfide loop and one small non-essential (C101-C104) disulfide loop | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
531 | - |
mutant G100K/E102P/S103G/G105W, pH 7.0 | Bos taurus |
962 | - |
mutant E102P/S103G, pH 7.0 | Bos taurus |
968 | - |
mutant G100K/G105W, pH 7.0 | Bos taurus |
1009 | - |
wild-type, pH 7.0 | Bos taurus |
1020 | - |
mutant G100W/E102/G/S103P/G105K, pH 7.0 | Bos taurus |
1092 | - |
mutant E102G/S103P, pH 7.0 | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
calf thymus DNA + H2O | - |
Bos taurus | 5'-phosphooligonucleotides + ? | - |
? |