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Literature summary for 3.1.21.1 extracted from

  • Mogi, K.; Takeshita, H.; Yasuda, T.; Nakajima, T.; Nakazato, E.; Kaneko, Y.; Itoi, M.; Kishi, K.
    Carp hepatopancreatic DNase I: biochemical, molecular, and immunological properties (2003), J. Biochem., 133, 377-386.
    View publication on PubMed
Search for more literature for 3.1.21.1

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in COS-7 cells Cyprinus carpio

Protein Variants

Protein Variants Comment Organism
F114A site-directed mutagenesis, change to human enzyme sequence type, complete abolished affinity for actin Cyprinus carpio
V68I site-directed mutagenesis, change to human enzyme sequence type, 90% reduced affinity for actin Cyprinus carpio

Inhibitors

Inhibitors Comment Organism Structure
EDTA complete inhibition at 20 mM Cyprinus carpio
G-actin slight inhibition Cyprinus carpio

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Cyprinus carpio 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Cyprinus carpio
Mg2+ activates Cyprinus carpio
Mn2+ activates Cyprinus carpio

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 x * 32000, SDS-PAGE Cyprinus carpio

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dsDNA + H2O Cyprinus carpio
-
 5'-phosphodinucleotide + 5'-phosphooligonucleotide
-
 ?
additional information Cyprinus carpio phylogenetic analysis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Cyprinus carpio Q8JIP7 carp
-

Purification (Commentary)

Purification (Comment) Organism
17000fold from hepatopancreas, to homogeneity Cyprinus carpio

Reaction

Reaction Comment Organism Reaction ID
endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products conserved residues Glu79, His135, Asp213, and His 252 are involved in catalysis Cyprinus carpio
a

Source Tissue

Source Tissue Comment Organism Textmining
gill filament low activity Cyprinus carpio
-
gill raker low activity Cyprinus carpio
-
heart low activity Cyprinus carpio
-
hepatopancreas highest enzyme expression and activity Cyprinus carpio
-
kidney low activity Cyprinus carpio
-
additional information no activity in brain Cyprinus carpio
-
pancreas
-
 Cyprinus carpio
-
small intestine
-
 Cyprinus carpio
-
spleen
-
 Cyprinus carpio
-
swim bladder
-
 Cyprinus carpio
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 purified enzyme Cyprinus carpio

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dsDNA + H2O
-
 Cyprinus carpio 5'-phosphodinucleotide + 5'-phosphooligonucleotide
-
 ?
additional information phylogenetic analysis Cyprinus carpio ?
-
 ?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Cyprinus carpio

Synonyms

Synonyms Comment Organism
DNase I
-
 Cyprinus carpio

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Cyprinus carpio