Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in COS-7 cells | Bufo japonicus |
DNA and amino acid sequence determination and analysis, expression in COS-7 cells | Cynops pyrrhogaster |
DNA and amino acid sequence determination and analysis, expression in COS-7 cells as His-tagged enzyme | Lithobates catesbeianus |
DNA and amino acid sequence determination and analysis, expression in COS-7 cells as His-tagged enzyme | Cynops pyrrhogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by G-actin | Bufo japonicus | |
additional information | no inhibition by G-actin; no inhibition by G-actin | Cynops pyrrhogaster | |
additional information | no inhibition by G-actin | Lithobates catesbeianus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | essential, binding site characterization | Lithobates catesbeianus | |
Ca2+ | essential, binding site characterization | Cynops pyrrhogaster | |
Ca2+ | essential, binding site characterization | Bufo japonicus | |
Mg2+ | - |
Lithobates catesbeianus | |
Mg2+ | - |
Cynops pyrrhogaster | |
Mg2+ | - |
Bufo japonicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
gel filtration | Lithobates catesbeianus |
40000 | - |
gel filtration | Cynops pyrrhogaster |
40000 | - |
gel filtration | Bufo japonicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dsDNA + H2O | Lithobates catesbeianus | - |
5'-phosphodinucleotide + 5'-phosphooligonucleotide | - |
? | |
dsDNA + H2O | Cynops pyrrhogaster | - |
5'-phosphodinucleotide + 5'-phosphooligonucleotide | - |
? | |
dsDNA + H2O | Bufo japonicus | - |
5'-phosphodinucleotide + 5'-phosphooligonucleotide | - |
? | |
additional information | Lithobates catesbeianus | phylogenetic analysis | ? | - |
? | |
additional information | Cynops pyrrhogaster | phylogenetic analysis | ? | - |
? | |
additional information | Bufo japonicus | phylogenetic analysis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bufo japonicus | Q90WM0 | - |
- |
Cynops pyrrhogaster | - |
- |
- |
Cynops pyrrhogaster | Q90W31 | - |
- |
Lithobates catesbeianus | Q90WM1 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | - |
Lithobates catesbeianus |
no glycoprotein | - |
Cynops pyrrhogaster |
no glycoprotein | - |
Bufo japonicus |
Purification (Comment) | Organism |
---|---|
1300fold from pancreas | Bufo japonicus |
1400fold from pancreas | Cynops pyrrhogaster |
1800fold from pancreas | Lithobates catesbeianus |
1800fold from pancreas | Cynops pyrrhogaster |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | no activity in cerebrum, heart, lung, liver, kidney, spleen, skin, muscle, oesophagus, stomach, duodenum, and large intestine | Lithobates catesbeianus | - |
additional information | no activity in cerebrum, heart, lung, liver, kidney, spleen, skin, muscle, oesophagus, stomach, duodenum, and large intestine | Cynops pyrrhogaster | - |
additional information | no activity in cerebrum, heart, lung, liver, kidney, spleen, skin, muscle, oesophagus, stomach, duodenum, and large intestine | Bufo japonicus | - |
pancreas | high activity | Lithobates catesbeianus | - |
pancreas | high activity | Cynops pyrrhogaster | - |
pancreas | high activity | Bufo japonicus | - |
small intestine | low activity | Lithobates catesbeianus | - |
small intestine | low activity | Cynops pyrrhogaster | - |
small intestine | low activity | Bufo japonicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
purified enzyme | Lithobates catesbeianus |
additional information | - |
purified enzyme | Cynops pyrrhogaster |
additional information | - |
purified enzyme | Bufo japonicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dsDNA + H2O | - |
Lithobates catesbeianus | 5'-phosphodinucleotide + 5'-phosphooligonucleotide | - |
? | |
dsDNA + H2O | - |
Cynops pyrrhogaster | 5'-phosphodinucleotide + 5'-phosphooligonucleotide | - |
? | |
dsDNA + H2O | - |
Bufo japonicus | 5'-phosphodinucleotide + 5'-phosphooligonucleotide | - |
? | |
additional information | phylogenetic analysis | Lithobates catesbeianus | ? | - |
? | |
additional information | phylogenetic analysis | Cynops pyrrhogaster | ? | - |
? | |
additional information | phylogenetic analysis | Bufo japonicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, SDS-PAGE | Lithobates catesbeianus |
monomer | 1 * 40000, SDS-PAGE | Cynops pyrrhogaster |
monomer | 1 * 40000, SDS-PAGE | Bufo japonicus |
More | structural analysis, insertion of a serine residue at position 205 and addition of a cysteine-rich C-stretch at the C-terminus are significant | Lithobates catesbeianus |
More | structural analysis, insertion of a serine residue at position 205 and addition of a cysteine-rich C-stretch at the C-terminus are significant | Cynops pyrrhogaster |
More | structural analysis, insertion of a serine residue at position 205 and addition of a cysteine-rich C-stretch at the C-terminus are significant | Bufo japonicus |
Synonyms | Comment | Organism |
---|---|---|
DNase I | - |
Lithobates catesbeianus |
DNase I | - |
Cynops pyrrhogaster |
More | enzyme belongs to the DNase I family | Lithobates catesbeianus |
More | enzyme belongs to the DNase I family | Cynops pyrrhogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
bell-shaped optimum | Lithobates catesbeianus |
8 | - |
bell-shaped optimum | Cynops pyrrhogaster |
8 | - |
bell-shaped optimum | Bufo japonicus |