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Literature summary for 3.1.21.1 extracted from

  • Funkakoshi, A.; Tsubota, Y.; Fujii, K.; Ibayashi, H.; Takagi, Y.
    Simple purification and properties of bovine pancreatic deoxyribonuclease I (1980), J. Biochem., 88, 1113-1118.
    View publication on PubMed
Search for more literature for 3.1.21.1

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ most active, faster production of linear form of supercoiled DNA Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded DNA + H2O Bos taurus
-
 5'-phosphooligonucleotides + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 4 active compounds: A, B, C, D
-

Purification (Commentary)

Purification (Comment) Organism
near homogeneity by isoelectric focusing and gel filtration Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 units defined as absorbance at 260 nm/ml assay solution Bos taurus
additional information
-
 320 units/mg protein Bos taurus

Storage Stability

Storage Stability Organism
0°C, 10 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 5 mM CaCl2, 30% polyethylene glycol, 4 weeks Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded DNA + H2O
-
 Bos taurus 5'-phosphooligonucleotides + ?
-
 ?
supercoiled plasmid DNA + H2O
-
 Bos taurus linear DNA + ? superhelical form converted to circular-relaxed and linear forms ?

Subunits

Subunits Comment Organism
monomer four active fractions, gel filtration, SDS-PAGE Bos taurus

Synonyms

Synonyms Comment Organism
DNase A
-
 Bos taurus
DNase B
-
 Bos taurus
DNase C
-
 Bos taurus
DNase D
-
 Bos taurus