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Literature summary for 3.1.21.1 extracted from

  • Lehman, I.R.
    Bacterial deoxyribonucleases (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 4, 251-270.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
RNA enhanced activity by pretreatment with ribonuclease, variety of RNA's including tRNA Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ absolute requirement for Mn2+ or Mg2+ Escherichia coli
Mg2+ absolute requirement for Mg2+ or Mn2+ Escherichia coli
Mn2+
-
Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded DNA + H2O Escherichia coli cleaves both strands of a double helix at or near the same level, about 400 nucleotides removed at each endonucleolytic scission, causes a "shattering" at the point of cleavage, leading to the libration of small oligonucleotides by an exonucleolytic mode of attack 5'-phosphooligonucleotides + ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Escherichia coli
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O degrades native DNA 7fold faster than denatured DNA Escherichia coli 5'-phosphooligonucleotides + ? products have an avarage chain length of 7 residues ?
double-stranded DNA + H2O cleaves both strands of a double helix at or near the same level, about 400 nucleotides removed at each endonucleolytic scission, causes a "shattering" at the point of cleavage, leading to the libration of small oligonucleotides by an exonucleolytic mode of attack Escherichia coli 5'-phosphooligonucleotides + ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
Escherichia coli