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Literature summary for 3.1.2.6 extracted from

  • Ridderstroem, M.; Jemth, P.; Cameron, A.D.; Mannervik, B.
    The active-site residue tyr-175 in human glyoxalase II contributes to binding of glutathione derivatives (2000), Biochim. Biophys. Acta, 1481, 344-348.
    View publication on PubMed
Search for more literature for 3.1.2.6

Protein Variants

Protein Variants Comment Organism
Y175F site-directed mutagenesis, mutation has a marginal effect on kcat, but leads to a 8fold increased Km for S-(2-hydroxyacyl)glutathione compared to the wild-type, mutant shows a 30fold increased sensitivity to inhibition by S-(N-hydroxy-N-bromophenylcarbamoyl)glutathione compared to the wild-type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
S-(N-hydroxy-N-bromophenylcarbamoyl)glutathione competitive inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 S-D-lactoylglutathione recombinant wild-type enzyme, pH 7.1, 37°C Homo sapiens
1.6
-
 S-D-lactoylglutathione recombinant mutant Y175F, pH 7.1, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate active site residue Tyr175 contributes to the binding of glutathione derivatives by its hydroxyl group which forms hydrogen binds to the glycine in the glutathione moiety Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-D-lactoylglutathione + H2O
-
 Homo sapiens glutathione + D-lactate
-
 ?

Synonyms

Synonyms Comment Organism
acetoacetylglutathione hydrolase
-
 Homo sapiens
Germ cell specific protein
-
 Homo sapiens
GLO II
-
 Homo sapiens
glyoxalase II
-
 Homo sapiens
hydrolase, acetoacetylglutathione
-
 Homo sapiens
hydrolase, hydroxyacylglutathione
-
 Homo sapiens
Round spermatid protein RSP29
-
 Homo sapiens
S-2-hydroxylacylglutathione hydrolase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
510
-
 S-D-lactoylglutathione recombinant mutant Y175F, pH 7.1, 37°C Homo sapiens
780
-
 S-D-lactoylglutathione recombinant wild-type enzyme, pH 7.1, 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
 assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0012
-
 S-(N-hydroxy-N-bromophenylcarbamoyl)glutathione recombinant wild-type enzyme, pH 7.1, 37°C Homo sapiens
0.035
-
 S-(N-hydroxy-N-bromophenylcarbamoyl)glutathione recombinant mutant Y175F, pH 7.1, 37°C Homo sapiens