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Literature summary for 3.1.2.6 extracted from

  • Bito, A.; Haider, M.; Briza, P.; Strasser, P.; Breitenbach, M.
    Heterologous expression, purification, and kinetic comparison of the cytoplasmic and mitochondrial glyoxalase II enzymes, Glo2p and Glo4p, from Saccharomyces cerevisiae (1999), Protein Expr. Purif., 17, 456-464.
    View publication on PubMed
Search for more literature for 3.1.2.6

Cloned(Commentary)

Cloned (Comment) Organism
cytoplasmic gene GLO2 and mitochondrial gene GLO4, expression in Escherichia coli Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Saccharomyces cerevisiae 5829
-
mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31153
-
 x * 31153, recombinant enzyme from mitochondria, GLO2, MALDI-TOF Saccharomyces cerevisiae
31321
-
 x * 31321, recombinant enzyme from cytosol, GLO2, MALDI-TOF Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-D-mandeloylglutathione + H2O
-
 Saccharomyces cerevisiae D-mandelate + glutathione
-
 ?
S-mandeloylglutathione + H2O
-
 Saccharomyces cerevisiae mandelate + glutathione
-
 ?

Subunits

Subunits Comment Organism
? x * 31153, recombinant enzyme from mitochondria, GLO2, MALDI-TOF Saccharomyces cerevisiae
? x * 31321, recombinant enzyme from cytosol, GLO2, MALDI-TOF Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
25.8
-
 S-D-mandeloylglutathione recombinant enzyme from mitochondria Saccharomyces cerevisiae
35.1
-
 S-D-mandeloylglutathione recombinant enzyme from cytosol Saccharomyces cerevisiae
38.1
-
 S-D-mandeloylglutathione wild-type enzyme from mitochondria Saccharomyces cerevisiae
605
-
 S-D-lactoylglutathione wild-type enzyme from mitochondria Saccharomyces cerevisiae
723
-
 S-D-lactoylglutathione recombinat enzyme from mitochondria Saccharomyces cerevisiae
979
-
 S-D-lactoylglutathione recombinat enzyme from cytosol Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 9 mitochondrial enzyme Saccharomyces cerevisiae
7 8 cytoplasmic enzyme Saccharomyces cerevisiae