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Literature summary for 3.1.2.6 extracted from

  • Ridderstroem, M.; Mannervik, B.
    Molecular cloning and charaterization of the thiolesterase glyoxalase II from Arabidopsis thaliana (1997), Biochem. J., 322, 449-454.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
(S)-D-mandeloylglutathione
-
Arabidopsis thaliana
0.098
-
S-D-lactoylglutathione
-
Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28791
-
x * 28791, calculation from nucleotide sequence Arabidopsis thaliana
29000
-
x * 29000, SDS-PAGE Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
large scale purification of recombinant enzyme Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-D-mandeloylglutathione + H2O
-
Arabidopsis thaliana D-mandelate + glutathione
-
?
lactoylglutathione + H2O
-
Arabidopsis thaliana D-lactate + glutathione
-
?
S-D-mandeloylglutathione + H2O
-
Arabidopsis thaliana D-mandelate + glutathione
-
?
S-mandeloylglutathione + H2O
-
Arabidopsis thaliana mandelate + glutathione
-
?

Subunits

Subunits Comment Organism
? x * 29000, SDS-PAGE Arabidopsis thaliana
? x * 28791, calculation from nucleotide sequence Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
74
-
S-D-mandeloylglutathione
-
Arabidopsis thaliana
466
-
S-D-lactoylglutathione
-
Arabidopsis thaliana