Cloned (Comment) | Organism |
---|---|
subcloning in Escherichia coli strain XL1-Blue, and expression in Escherichia coli strain BL21 (DE3) under control of the T7 promoter, co-expression with the beta-ketothiolase gene from Ralstonia eutropha strain H16 and the (S)-3-hydroxybutyryl-CoA dehydrogenase gene from Ralstonia eutropha strain H16, or Clostridium acetobutylicum strain ATCC824 leads to biosynthesis of enantiomerically pure (S)-3-hydroxybutyric acid in the engineered Escherichia coli cells, extracellular product, method optimization, overview | Bacillus cereus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-2-methylpropanoyl-CoA + H2O | Bacillus cereus | - |
CoA + 3-hydroxy-2-methylpropanoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | Q81DR3 | strain ATCC 14579 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.073 | - |
recombinant BCH in Escherichia coli strain BL21(DE3) crude extract | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-2-methylpropanoyl-CoA + H2O | - |
Bacillus cereus | CoA + 3-hydroxy-2-methylpropanoate | - |
? | |
3-hydroxy-2-methylpropanoyl-CoA + H2O | - |
Bacillus cereus | CoA + 3-hydroxy-2-methylpropanoate | (S)-3-hydroxyacids and their esters, such as (S)-3-hydroxybutyric acid, can be used as chiral building blocks for the chemical synthesis of optically active fine chemicals such as antibiotics, vitamins, perfumes, and pheromones | ? |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxyisobutyryl-CoA hydrolase | - |
Bacillus cereus |
BCH | - |
Bacillus cereus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Bacillus cereus |