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Literature summary for 3.1.2.32 extracted from

  • Folch, B.; Deziel, E.; Doucet, N.
    Systematic mutational analysis of the putative hydrolase PqsE: toward a deeper molecular understanding of virulence acquisition in Pseudomonas aeruginosa (2013), PLoS ONE, 8, e73727.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.2.32

Protein Variants

Protein Variants Comment Organism
D130A mutant in KH-like motif, pyocyanin production 0.2 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
D178A active site mutant, pyocyanin production 0.1 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
D73A active site mutant, pyocyanin production 4.1 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
E182A active site mutant, pyocyanin production 1.2 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
F195A active site mutant, pyocyanin production 0.8 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
F276A active site mutant, pyocyanin production 0.1 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
H159A active site mutant, pyocyanin production 0.1 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
H221A active site mutant, pyocyanin production 1.1 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
H282A C-terminal alpha-helix mutant, pyocyanin production 0.4 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
H69A active site mutant, pyocyanin production 0.1 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
H71A active site mutant, pyocyanin production 2.7 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
H74A active site mutant, pyocyanin production 3.5 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
K101A mutant in KH-like motif, pyocyanin production 4.4 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
L193A active site mutant, pyocyanin production 4.7 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
L248A C-terminal alpha-helix mutant, pyocyanin production 4.1 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
L261A C-terminal alpha-helix mutant, pyocyanin production 0.2 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
L277A active site mutant, pyocyanin production 4.5 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
L290A C-terminal alpha-helix mutant, pyocyanin production 0.1 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
M286A C-terminal alpha-helix mutant, pyocyanin production 0.1 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
Q272A active site mutant, pyocyanin production 1.4 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
R107A mutant in KH-like motif, pyocyanin production 4.0 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
R111A mutant in KH-like motif, pyocyanin production 0.3 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
R288A active site mutant, pyocyanin production 5.2 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
R95A mutant in KH-like motif, pyocyanin production 5.1 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
S273A active site mutant, pyocyanin production 0.1 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
S285A active site mutant, pyocyanin production 5.0 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
S285W active site mutant, pyocyanin production 5.6 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa
W269A C-terminal alpha-helix mutant, pyocyanin production 0.1 mg/l compared with 6.2 mg/l for wild-type Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa A0A0H2Z6F6
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Pseudomonas aeruginosa UCBPP-PA14 A0A0H2Z6F6
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General Information

General Information Comment Organism
physiological function the active site of the enzyme involves residues that extend further into the hydrophobic core of the protein, advocating for a lid-like movement of the two terminal helices. A unique C-terminal alpha-helical motif is important for the molecular function Pseudomonas aeruginosa