Literature summary for 3.1.2.28 extracted from

Furt, F.; Allen, W.J.; Widhalm, J.R.; Madzelan, P.; Rizzo, R.C.; Basset, G.; Wilson, M.A.
Functional convergence of structurally distinct thioesterases from cyanobacteria and plants involved in phylloquinone biosynthesis (2013), Acta Crystallogr. Sect. D, 69, 1876-1888.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Synechocystis sp.
expression in Escherichia coli	Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment)	Organism
to 1.9 A resolution. The core structure is defined by a 5-6-stranded antiparallel beta-sheet that partially wraps around a prominent five-turn alpha-helix. Isoform DHNAT1 has a 27-amino-acid extension at its N-terminus. DHNAT1 is a facial tetramer that features a dimer-dimer interface near the hotdog alpha-helices, which point inward towards the center of the tetramer. The binding site for substrate 1,4-dihydroxy-2-naphthoyl-CoA spans the dimer interface	Arabidopsis thaliana
to 1.9 A resolution. The core structure is defined by a 5-6-stranded antiparallel beta-sheet that partially wraps around a prominent five-turn alpha-helix. Isoform Slr0204 has a 25-amino-acid extension at its C-terminus, residues 113-138. Slr0204 is a facial tetramer that features a dimer-dimer interface near the hotdog alpha-helices, which point inward towards the center of the tetramer. The binding site for substrate 1,4-dihydroxy-2-naphthoyl-CoA spans the dimer interface	Synechocystis sp.

Crystallization (Comment)

Organism

to 1.9 A resolution. The core structure is defined by a 5-6-stranded antiparallel beta-sheet that partially wraps around a prominent five-turn alpha-helix. Isoform DHNAT1 has a 27-amino-acid extension at its N-terminus. DHNAT1 is a facial tetramer that features a dimer-dimer interface near the hotdog alpha-helices, which point inward towards the center of the tetramer. The binding site for substrate 1,4-dihydroxy-2-naphthoyl-CoA spans the dimer interface

Arabidopsis thaliana

to 1.9 A resolution. The core structure is defined by a 5-6-stranded antiparallel beta-sheet that partially wraps around a prominent five-turn alpha-helix. Isoform Slr0204 has a 25-amino-acid extension at its C-terminus, residues 113-138. Slr0204 is a facial tetramer that features a dimer-dimer interface near the hotdog alpha-helices, which point inward towards the center of the tetramer. The binding site for substrate 1,4-dihydroxy-2-naphthoyl-CoA spans the dimer interface

Synechocystis sp.

Protein Variants

Protein Variants	Comment	Organism
D16N	active site mutant, modeling of substrate bound to active site	Synechocystis sp.
E57Q	active site mutant, modeling of substrate bound to active site	Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
15700	-	4 * 15700, calculated	Synechocystis sp.
17000	-	4 * 17000, calculated	Arabidopsis thaliana
62500	-	sedimentation-equilibrium ultracentrifugation	Arabidopsis thaliana
77400	-	sedimentation-equilibrium ultracentrifugation	Synechocystis sp.

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9SX65	-	-
Synechocystis sp.	Q55777	-	-

Subunits

Subunits	Comment	Organism
tetramer	4 * 15700, calculated	Synechocystis sp.
tetramer	4 * 17000, calculated	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
AtDHNAT1	-	Arabidopsis thaliana
DHNA-CoA thioesterase	-	Synechocystis sp.
DHNA-CoA thioesterase	-	Arabidopsis thaliana
slr0204	-	Synechocystis sp.