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Literature summary for 3.1.2.27 extracted from
- Presence of choloyl- and chenodeoxycholoyl-coenzyme A thioesterase activity in human liver (2000), Scand. J. Clin. Lab. Invest., 60, 91-102.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| CoA | slight activation of activity with chenodeoxycholoyl-CoA | Homo sapiens |  |
| DTT | highly stimulating on the activity with both choloyl-CoA and chenodeoxycholoyl-CoA | Homo sapiens | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CoA | slight inhibition of activity with choloyl-CoA | Homo sapiens | |
| DTNB | 75% inhibition at 0.3 mM, complete inhibition at 1.3 mM | Homo sapiens | |
| additional information | substrate competition between choloyl-CoA and chenodeoxycholoyl-CoA, overview | Homo sapiens | |
| Phospholipids | at high concentrations | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Homo sapiens | |
| 0.011 | - |
dithiothreitol | pH 8.0, 37°C, with chenodeoxycholoyl-CoA | Homo sapiens | |
| 0.018 | - |
dithiothreitol | pH 8.0, 37°C, with choloyl-CoA | Homo sapiens | |
| 0.071 | - |
chenodeoxycholoyl-CoA | pH 8.0, 37°C, postnuclear fraction of liver homogenate | Homo sapiens | |
| 0.077 | - |
choloyl-CoA | pH 8.0, 37°C, postnuclear fraction of liver homogenate | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
| microsome | - |
Homo sapiens | - |
- |
| mitochondrion | - |
Homo sapiens | 5739 | - |
| additional information | multiorganelle distribution, overview | Homo sapiens | - |
- |
| nucleus | - |
Homo sapiens | 5634 | - |
| peroxisome | - |
Homo sapiens | 5777 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chenodeoxycholoyl-CoA + H2O | Homo sapiens | - |
chenodeoxycholate + CoA | free chenodeoxycholate activates the farnesoid X receptor | ? | |
| choloyl-CoA + H2O | Homo sapiens | - |
cholate + CoA | - |
? | |
| additional information | Homo sapiens | the enzyme has a regulatory function in bile acid metabolism | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially, subcellular fractionation | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| choloyl-CoA + H2O = cholate + CoA | the catalytic triad is formed by Asp233, Ser255, and Gln305 | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
| liver | healthy and metastatic | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
| 0.00118 | - |
nuclear fraction of liver homogenate, substrate choloyl-CoA | Homo sapiens |
| 0.00127 | - |
cytosolic fraction of liver homogenate, substrate choloyl-CoA | Homo sapiens |
| 0.00186 | - |
nuclear fraction of liver homogenate, substrate chenodeoxycholoyl-CoA | Homo sapiens |
| 0.00203 | - |
microsomal fraction of liver homogenate, substrate chenodeoxycholoyl-CoA | Homo sapiens |
| 0.00203 | - |
mitochondrial fraction of liver homogenate, substrate choloyl-CoA | Homo sapiens |
| 0.00227 | - |
cytosolic fraction of liver homogenate, substrate chenodeoxycholoyl-CoA | Homo sapiens |
| 0.00247 | - |
light mitochondrial fraction of liver homogenate, substrate choloyl-CoA | Homo sapiens |
| 0.00248 | - |
microsomal fraction of liver homogenate, substrate choloyl-CoA | Homo sapiens |
| 0.00263 | - |
mitochondrial fraction of liver homogenate, substrate chenodeoxycholoyl-CoA | Homo sapiens |
| 0.00276 | - |
postnuclear fraction of liver homogenate, substrate choloyl-CoA | Homo sapiens |
| 0.00297 | - |
postnuclear fraction of liver homogenate, substrate chenodeoxycholoyl-CoA | Homo sapiens |
| 0.00334 | - |
light mitochondrial fraction of liver homogenate, substrate chenodeoxycholoyl-CoA | Homo sapiens |
| 0.0133 | - |
peroxisomal fraction of a metastatic liver homogenate, substrate chenodeoxycholoyl-CoA | Homo sapiens |
| 0.01602 | - |
peroxisomal fraction of a metastatic liver homogenate, substrate choloyl-CoA | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chenodeoxycholoyl-CoA + H2O | - |
Homo sapiens | chenodeoxycholate + CoA | - |
? | |
| chenodeoxycholoyl-CoA + H2O | - |
Homo sapiens | chenodeoxycholate + CoA | free chenodeoxycholate activates the farnesoid X receptor | ? | |
| chenodeoxycholoyl-CoA + H2O | preferred substrate | Homo sapiens | chenodeoxycholate + CoA | - |
? | |
| choloyl-CoA + H2O | - |
Homo sapiens | cholate + CoA | - |
? | |
| additional information | the enzyme has a regulatory function in bile acid metabolism | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| chenodeoxycholic acid-CoA thioesterase | - |
Homo sapiens |
| chenodeoxycholoyl-coenzyme A thioesterase | - |
Homo sapiens |
| choloyl-coenzyme A thioesterase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | broad optimum with substrate choloyl-CoA | Homo sapiens |
| 7.5 | 9 | broad optimum with substrate chenodeoxycholoyl-CoA | Homo sapiens |
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