BRENDA - Enzyme Database
show all sequences of 3.1.2.23

Structure-activity analysis of base and enzyme-catalyzed 4-hydroxybenzoyl coenzyme A hydrolysis

Song, F.; Zhuang, Z.; Dunaway-Mariano, D.; Bioorg. Chem. 35, 1-10 (2007)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.006
-
4-hydroxybenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
5
20
4-fluorobenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
56
-
4-methoxybenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
230
-
4-methylbenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
300
-
4-trifluoromethylbenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
510
-
benzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
550
-
4-chlorobenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
P56653
CBS3, recombinantly expressed in Escherichia coli
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-Chlorobenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-Chlorobenzoate + CoA
-
-
-
?
4-fluorobenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-fluorobenzoate + CoA
-
-
-
?
4-Hydroxybenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-Hydroxybenzoate + CoA
-
-
-
?
4-methoxybenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-methoxybenzoate + CoA
-
-
-
?
4-methylbenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-methylbenzoate + CoA
-
-
-
?
4-trifluoromethylbenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-trifluoromethylbenzoate + CoA
-
-
-
?
Benzoyl-CoA + H2O
-
678580
Pseudomonas sp.
Benzoate + CoA
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.038
-
4-trifluoromethylbenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
0.047
-
4-methylbenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
0.27
-
4-methoxybenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
1
-
benzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
1.1
-
4-chlorobenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
9.7
-
4-fluorobenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
18.3
-
4-hydroxybenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.006
-
4-hydroxybenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
5
20
4-fluorobenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
56
-
4-methoxybenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
230
-
4-methylbenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
300
-
4-trifluoromethylbenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
510
-
benzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
550
-
4-chlorobenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-Chlorobenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-Chlorobenzoate + CoA
-
-
-
?
4-fluorobenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-fluorobenzoate + CoA
-
-
-
?
4-Hydroxybenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-Hydroxybenzoate + CoA
-
-
-
?
4-methoxybenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-methoxybenzoate + CoA
-
-
-
?
4-methylbenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-methylbenzoate + CoA
-
-
-
?
4-trifluoromethylbenzoyl-CoA + H2O
-
678580
Pseudomonas sp.
4-trifluoromethylbenzoate + CoA
-
-
-
?
Benzoyl-CoA + H2O
-
678580
Pseudomonas sp.
Benzoate + CoA
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.038
-
4-trifluoromethylbenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
0.047
-
4-methylbenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
0.27
-
4-methoxybenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
1
-
benzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
1.1
-
4-chlorobenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
9.7
-
4-fluorobenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
18.3
-
4-hydroxybenzoyl-CoA
pH 7.5, 25°C
Pseudomonas sp.
Other publictions for EC 3.1.2.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729220
Song
The catalytic mechanism of the ...
Arthrobacter sp., Arthrobacter sp. SU / DSM 20407
Biochemistry
51
7000-7016
2012
-
-
-
1
24
-
-
18
-
-
-
-
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
23
-
-
-
-
-
-
-
-
-
-
-
1
24
-
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
23
-
-
-
-
-
-
-
-
20
20
729223
Zhuang
Investigation of the catalytic ...
Pseudomonas sp., Pseudomonas sp. CBS-3
Biochemistry
51
786-794
2012
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
707108
Li
Monitoring enzyme catalysis in ...
Arthrobacter sp., Arthrobacter sp. SU / DSM 20407
Anal. Biochem.
394
209-216
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690642
Ismail
Benzoyl-coenzyme A thioesteras ...
Azoarcus evansii
Arch. Microbiol.
190
451-460
2008
-
-
1
-
-
-
4
2
-
1
2
10
-
3
-
-
1
-
-
-
2
1
10
1
1
-
1
2
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
2
-
1
2
10
-
-
-
1
-
-
2
1
10
1
1
-
1
2
1
2
-
-
-
-
-
-
-
-
678580
Song
Structure-activity analysis of ...
Pseudomonas sp.
Bioorg. Chem.
35
1-10
2007
-
-
-
-
-
-
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
7
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
652473
Thoden
The structure of 4-hydroxybenz ...
Arthrobacter sp., Arthrobacter sp. SU / DSM 20407
J. Biol. Chem.
278
43709-43716
2003
-
-
-
1
-
-
2
1
-
-
-
2
-
9
-
-
-
1
-
-
-
-
4
1
1
-
-
-
1
-
-
-
2
-
-
-
-
-
-
1
-
-
-
2
2
1
-
-
-
2
-
-
-
-
-
-
-
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
650103
Zhuang
Kinetic, Raman, NMR, and site- ...
Pseudomonas sp., Pseudomonas sp. CBS3
Biochemistry
41
11152-11160
2002
-
-
-
-
8
-
1
7
-
-
-
2
-
10
-
-
-
1
-
-
-
-
2
-
-
-
-
8
-
-
-
-
6
-
-
-
-
-
-
-
8
-
-
1
6
7
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
652279
Thoden
X-ray crystallographic analyse ...
Pseudomonas sp., Pseudomonas sp. CBS-3
J. Biol. Chem.
277
27468-27476
2002
-
-
-
1
1
-
2
1
-
-
-
2
-
11
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
2
-
1
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
23921
Benning
The three-dimensional structur ...
Pseudomonas sp., Pseudomonas sp. CBS-3
J. Biol. Chem.
273
33572-33579
1998
-
-
-
-
-
-
-
-
-
-
-
2
-
12
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
781
Dunaway-Mariano
On the origins and functions o ...
Arthrobacter sp., Arthrobacter sp. SU / DSM 20407, Pseudomonas sp., Pseudomonas sp. CBS-3
Biodegradation
5
259-276
1994
-
-
1
-
-
-
-
-
-
-
2
-
-
17
-
-
-
-
-
-
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
780
Chang
Isolation and characterization ...
Pseudomonas sp., Pseudomonas sp. CBS-3
Biochemistry
31
5605-5610
1992
-
-
1
-
-
-
-
3
-
-
3
-
-
10
-
-
1
-
-
-
1
-
6
1
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
3
-
-
-
-
1
-
-
1
-
6
1
-
-
-
3
-
-
-
-
-
-
-
-
-
-