Literature summary for 3.1.2.23 extracted from

Thoden, J.B.; Zhuang, Z.; Dunaway-Mariano, D.; Holden, H.M.
The structure of 4-hydroxybenzoyl-CoA thioesterase from arthrobacter sp. strain SU (2003), J. Biol. Chem., 278, 43709-43716.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant wild-type enzyme with and without bound inhibitor, hanging drop vapour diffusion method, 4°C, 18 mg/ml protein solution containing 10 mM HEPES, pH 7.5, 150 mM KCl, 1 mM 1,4-dithio-D,L-threitol, with or without 1 mM 4-hydroxyphenacyl-CoA, plus precipitant solution containing 17-20% PEG 3400, 100 mM MOPS, pH 7.0, and 200 mM LiCl, X-ray diffraction structure determination at 2.6 A resolution, and analysis	Arthrobacter sp.

Crystallization (Comment)

Organism

purified recombinant wild-type enzyme with and without bound inhibitor, hanging drop vapour diffusion method, 4°C, 18 mg/ml protein solution containing 10 mM HEPES, pH 7.5, 150 mM KCl, 1 mM 1,4-dithio-D,L-threitol, with or without 1 mM 4-hydroxyphenacyl-CoA, plus precipitant solution containing 17-20% PEG 3400, 100 mM MOPS, pH 7.0, and 200 mM LiCl, X-ray diffraction structure determination at 2.6 A resolution, and analysis

Arthrobacter sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
4-hydroxybenzyl-CoA	-	Arthrobacter sp.
4-hydroxyphenacyl-CoA	binding mechanism	Arthrobacter sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics	Arthrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4-Hydroxybenzoyl-CoA + H2O	Arthrobacter sp.	enzyme is part of the dehalogenation pathway of 4-chlorobenzoyl-CoA in soil bacteria	4-Hydroxybenzoate + CoA	-	?
4-Hydroxybenzoyl-CoA + H2O	Arthrobacter sp. SU / DSM 20407	enzyme is part of the dehalogenation pathway of 4-chlorobenzoyl-CoA in soil bacteria	4-Hydroxybenzoate + CoA	-	?

Organism

Organism	UniProt	Comment	Textmining
Arthrobacter sp.	-	-	-
Arthrobacter sp. SU / DSM 20407	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
4-Hydroxybenzoyl-CoA + H2O = 4-hydroxybenzoate + CoA	active site structure and ligand binding mechanism, overview	Arthrobacter sp.	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
4-Hydroxybenzoyl-CoA + H2O	-	Arthrobacter sp.	4-Hydroxybenzoate + CoA	-	?
4-Hydroxybenzoyl-CoA + H2O	enzyme is part of the dehalogenation pathway of 4-chlorobenzoyl-CoA in soil bacteria	Arthrobacter sp.	4-Hydroxybenzoate + CoA	-	?
4-Hydroxybenzoyl-CoA + H2O	-	Arthrobacter sp. SU / DSM 20407	4-Hydroxybenzoate + CoA	-	?
4-Hydroxybenzoyl-CoA + H2O	enzyme is part of the dehalogenation pathway of 4-chlorobenzoyl-CoA in soil bacteria	Arthrobacter sp. SU / DSM 20407	4-Hydroxybenzoate + CoA	-	?

Subunits

Subunits	Comment	Organism
tetramer	dimer of dimers, structure analysis	Arthrobacter sp.

Synonyms

Synonyms	Comment	Organism
Hydrolase, 4-hydroxybenzoyl coenzyme A	-	Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Arthrobacter sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Arthrobacter sp.

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0000003	-	4-hydroxyphenacyl-CoA	pH 7.5, 25°C	Arthrobacter sp.
0.0006	-	4-hydroxybenzyl-CoA	pH 7.5, 25°C	Arthrobacter sp.