Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant in COS-1 cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme with and without bound palmitate, sitting drop vapor diffusion method, 12 mg/ml enzyme in 20 mM HEPES, pH 7.0, plus 150 mM NaCl, plus reservoir solution, containing 55% PEG 400, 100 mM Bis-Tris, pH 6.5, in a ratio 3:2, equilibrated against a250fold excess, X-ray multiwave length anomalous diffraction phasing for structure determinationand analysis, 2.25 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D233N | site-directed mutagenesis, inactive mutant | Homo sapiens |
D79G | site-directed mutagenesis, highly reduced activity | Homo sapiens |
H289A | site-directed mutagenesis, inactive mutant | Homo sapiens |
N197Q | site-directed mutagenesis, mutation of a glycosylation site, slightly reduced activity | Homo sapiens |
N197Q/N212Q | site-directed mutagenesis, mutation of glycosylation sites, slightly reduced activity, mutant shows 10% of the wild-type expression level | Homo sapiens |
N197Q/N212Q/N232Q | site-directed mutagenesis, mutation of all glycosylation sites, inactive mutant | Homo sapiens |
N197Q/N232Q | site-directed mutagenesis, mutation of glycosylation sites, reduced activity | Homo sapiens |
N212Q | site-directed mutagenesis, mutation of a glycosylation site, slightly reduced activity | Homo sapiens |
N212Q/N232Q | site-directed mutagenesis, mutation of glycosylation sites, highly reduced activity | Homo sapiens |
N232Q | site-directed mutagenesis, mutation of a glycosylation site, slightly reduced activity | Homo sapiens |
S115A | site-directed mutagenesis, inactive mutant | Homo sapiens |
T75P | site-directed mutagenesis, highly reduced activity | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Homo sapiens | 5764 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | enzyme deficiency causes progressive neurological disorder infantile neuronal ceroid lipofuscinosis, INCL, molecular basis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N-glycosylation at Asn232, Asn197, and Asn212, essential for activity | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
palmitoyl[protein] + H2O = palmitate + protein | specific for long-chain thioesters of fatty acids from S-acylated residues in proteins, palmitoyl cysteine and palmitoyl-CoA, catalytic triad consists of Ser115, His289, and Asp233 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme deficiency causes progressive neurological disorder infantile neuronal ceroid lipofuscinosis, INCL, molecular basis | Homo sapiens | ? | - |
? | |
palmitoyl-[protein] + H2O | - |
Homo sapiens | palmitate + protein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
palmitoyl protein thioesterase | - |
Homo sapiens |