BRENDA - Enzyme Database
show all sequences of 3.1.2.22

The crystal structure of palmitoyl protein thioesterase-2 (PPT2) reveals the basis for divergent substrate specificities of the two lysosomal thioesterases, PPT1 and PPT2

Calero, G.; Gupta, P.; Nonato, M.C.; Tandel, S.; Biehl, E.R.; Hofmann, S.L.; Clardy, J.; J. Biol. Chem. 278, 37957-37964 (2003)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
overexpression of PPT2 in an insect-baculovirus expression system, secretion of the recombinant enzyme to the culture medium, expression of mutant PPT2 in COS cells, subcloning in Escherichia coli XL1-blue
Homo sapiens
Crystallization (Commentary)
Crystallization (Commentary)
Organism
purified recombinant PPT2 15 mg/ml, sitting drop vapour diffusion method, 4C, with equal volume of precipitant solution conataining 2 M ammonium sulfate, 100 mM sodium cacodylate, pH 5.5-6.5, 8% methyl pentanediol, X-ray diffraction structure determination and analysis at 2.7 A resolution
Homo sapiens
Engineering
Protein Variants
Commentary
Organism
additional information
residues involved in ligand binding differ between PPT1 and PPT2, exchange by site-directed mutagenesis and functional analysis, overview
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
lysosome
PPT1 and PPT2
Homo sapiens
5764
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Homo sapiens
PPT1 deficiency causes progressive neurological disorder infantile neuronal ceroid lipofuscinosis, INCL
?
-
Homo sapiens
?
Organism
Organism
UniProt
Commentary
Textmining
Homo sapiens
-
2 lysosomal enzyme forms PPT1 and PPT2
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant PPT2 from overexpressing insect cells, recombinant PPT2 mutant from COS cells
Homo sapiens
Reaction
Reaction
Commentary
Organism
Reaction ID
palmitoyl[protein] + H2O = palmitate + protein
specific for long-chain thioesters of fatty acids from S-acylated residues in proteins, palmitoyl cysteine and palmitoyl-CoA, catalytic triad of PPT2 consists of Ser111, His283, and Asp228, mechanism, both PPT1 and PPT2 have lipid-binding grooves leading away from the active site
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-methylumbelliferyl 6-S-palmitoyl-6-thio-beta-D-glucoside + H2O
-
652459
Homo sapiens
4-methylumbelliferyl 6-thio-beta-D-glucoside + palmitate
-
652459
Homo sapiens
?
additional information
structural differences in the 2 enzyme fomrs PPT1 and PPT2, PPT2 does not hydrolyze substrates with bulky head groups
652459
Homo sapiens
?
-
652459
Homo sapiens
?
additional information
PPT1 deficiency causes progressive neurological disorder infantile neuronal ceroid lipofuscinosis, INCL
652459
Homo sapiens
?
-
652459
Homo sapiens
?
palmitoyl-CoA + H2O
preferred substrate of PPT2
652459
Homo sapiens
palmitate + CoA
-
652459
Homo sapiens
?
S-palmitoyl-N-acetyl-O-carboxymethyl-cysteine + H2O
enzyme form PPT1, not PPT2
652459
Homo sapiens
N-acetyl-O-carboxymethyl-cysteine + palmitate
-
652459
Homo sapiens
?
S-palmitoyl-N-acetylcysteamine + H2O
enzyme form PPT1, not PPT2
652459
Homo sapiens
N-acetylcysteamine + palmitate
-
652459
Homo sapiens
?
Synonyms
Synonyms
Commentary
Organism
palmitoyl protein thioesterase-1
-
Homo sapiens
palmitoyl protein thioesterase-2
-
Homo sapiens
PPT1
-
Homo sapiens
PPT2
-
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.2
-
assay at
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression of PPT2 in an insect-baculovirus expression system, secretion of the recombinant enzyme to the culture medium, expression of mutant PPT2 in COS cells, subcloning in Escherichia coli XL1-blue
Homo sapiens
Crystallization (Commentary) (protein specific)
Crystallization
Organism
purified recombinant PPT2 15 mg/ml, sitting drop vapour diffusion method, 4C, with equal volume of precipitant solution conataining 2 M ammonium sulfate, 100 mM sodium cacodylate, pH 5.5-6.5, 8% methyl pentanediol, X-ray diffraction structure determination and analysis at 2.7 A resolution
Homo sapiens
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
residues involved in ligand binding differ between PPT1 and PPT2, exchange by site-directed mutagenesis and functional analysis, overview
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
lysosome
PPT1 and PPT2
Homo sapiens
5764
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Homo sapiens
PPT1 deficiency causes progressive neurological disorder infantile neuronal ceroid lipofuscinosis, INCL
?
-
Homo sapiens
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant PPT2 from overexpressing insect cells, recombinant PPT2 mutant from COS cells
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-methylumbelliferyl 6-S-palmitoyl-6-thio-beta-D-glucoside + H2O
-
652459
Homo sapiens
4-methylumbelliferyl 6-thio-beta-D-glucoside + palmitate
-
652459
Homo sapiens
?
additional information
structural differences in the 2 enzyme fomrs PPT1 and PPT2, PPT2 does not hydrolyze substrates with bulky head groups
652459
Homo sapiens
?
-
652459
Homo sapiens
?
additional information
PPT1 deficiency causes progressive neurological disorder infantile neuronal ceroid lipofuscinosis, INCL
652459
Homo sapiens
?
-
652459
Homo sapiens
?
palmitoyl-CoA + H2O
preferred substrate of PPT2
652459
Homo sapiens
palmitate + CoA
-
652459
Homo sapiens
?
S-palmitoyl-N-acetyl-O-carboxymethyl-cysteine + H2O
enzyme form PPT1, not PPT2
652459
Homo sapiens
N-acetyl-O-carboxymethyl-cysteine + palmitate
-
652459
Homo sapiens
?
S-palmitoyl-N-acetylcysteamine + H2O
enzyme form PPT1, not PPT2
652459
Homo sapiens
N-acetylcysteamine + palmitate
-
652459
Homo sapiens
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.2
-
assay at
Homo sapiens
Other publictions for EC 3.1.2.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750869
Spinelli
-
Nutrient-dependent changes of ...
Homo sapiens
Int. J. Mol. Sci.
19
E3820
2018
-
-
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2
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1
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1
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7
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751433
Shyng
Widespread expression of a me ...
Homo sapiens
JIMD Rep.
36
85-92
2017
-
-
1
-
-
-
-
-
1
-
-
1
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1
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1
3
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1
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751466
Meyer
Decreased sensitivity of palm ...
Mus musculus
Metab. Brain Dis.
32
275-279
2017
-
-
-
-
-
-
-
-
1
-
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1
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3
-
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2
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2
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3
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2
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1
1
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751572
Dearborn
Histochemical localization of ...
Mus musculus
Mol. Genet. Metab.
117
210-216
2016
-
-
-
-
-
-
-
-
1
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1
-
5
-
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5
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2
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2
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5
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2
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-
-
-
-
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-
-
-
-
751937
Segal-Salto
Reversible cysteine acylation ...
Homo sapiens
PLoS ONE
11
e0146466
2016
-
-
1
-
1
-
-
-
3
-
-
-
-
2
-
1
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1
1
2
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1
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1
1
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751407
Scifo
Proteomic analysis of the pal ...
Homo sapiens
J. Proteomics
123
42-53
2015
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1
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2
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1
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1
3
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3
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2
1
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-
-
-
-
729768
Aby
Mutations in palmitoyl-protein ...
Drosophila melanogaster
Fly
7
267-279
2014
-
-
1
-
2
-
-
-
1
-
-
-
-
4
-
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2
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2
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-
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1
1
-
-
-
730430
Liu
Palmitoyl-protein thioesterase ...
Rattus norvegicus
Mol. Reprod. Dev.
81
55-65
2014
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
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3
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3
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1
-
-
1
-
-
730399
Hu
Intravenous high-dose enzyme r ...
Mus musculus
Mol. Genet. Metab.
107
213-221
2012
-
1
-
-
-
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1
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4
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2
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1
1
-
-
-
709975
Lu
Human recombinant palmitoyl-pr ...
Homo sapiens
Mol. Genet. Metab.
99
374-378
2010
-
1
1
-
-
-
-
-
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4
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1
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2
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1
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1
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2
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1
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-
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1
1
-
-
-
710070
Saja
Identifying cellular pathways ...
Drosophila melanogaster, Drosophila sp. (in: Insecta)
Neurobiol. Dis.
40
135-145
2010
1
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2
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4
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1
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3
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3
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3
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2
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2
2
-
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716234
Sanders
A mutation in canine PPT1 caus ...
Canis lupus familiaris
Mol. Genet. Metab.
100
349-356
2010
-
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4
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1
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1
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1
1
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716683
Chu-LaGraff
The Batten disease Palmitoyl P ...
Drosophila melanogaster
PLoS ONE
5
e14402
2010
-
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1
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4
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1
1
-
-
-
707548
Hirano
Thioesterase activity and subc ...
Homo sapiens
Biochim. Biophys. Acta
1791
797-805
2009
-
-
1
-
1
-
2
2
1
1
1
-
-
2
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1
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2
1
2
1
2
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1
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1
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1
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2
2
2
1
1
1
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1
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2
1
2
1
-
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1
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707557
Tardy
Palmitoyl protein thioesterase ...
Homo sapiens, Mus musculus
Biochim. Biophys. Acta
1793
1250-1258
2009
-
-
1
-
-
-
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4
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2
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2
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4
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2
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2
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4
4
-
-
-
708433
Macauley
Cerebellar pathology and motor ...
Mus musculus
Exp. Neurol.
217
124-135
2009
-
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2
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2
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2
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1
1
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692414
Bannan
The Drosophila protein palmito ...
Drosophila melanogaster
Fly
2
198-214
2008
-
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1
-
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1
-
2
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1
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3
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1
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693353
Kim
Palmitoyl protein thioesterase ...
Homo sapiens, Mus musculus
J. Clin. Invest.
118
3075-3086
2008
-
-
1
-
-
-
-
-
6
-
-
4
-
4
-
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4
-
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4
-
4
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1
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6
-
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4
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4
-
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4
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-
707922
Takano
Infantile neuronal ceroid lipo ...
Homo sapiens
Brain Dev.
30
370-373
2008
-
1
-
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2
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1
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