Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Streptomyces chartreusis | |
Fe2+ | almost complete inhibition at 1 mM | Streptomyces chartreusis | |
Mg2+ | - |
Streptomyces chartreusis | |
Mn2+ | almost complete inhibition at 10 mM | Streptomyces chartreusis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.141 | - |
palmitoyl-CoA | at pH 7.5 and 30°C | Streptomyces chartreusis | |
0.188 | - |
malonyl-CoA | at pH 7.5 and 30°C | Streptomyces chartreusis | |
0.19 | - |
butyryl-CoA | at pH 7.5 and 30°C | Streptomyces chartreusis | |
0.2 | - |
acetyl-CoA | at pH 7.5 and 30°C | Streptomyces chartreusis | |
0.216 | - |
benzoyl-CoA | at pH 7.5 and 30°C | Streptomyces chartreusis | |
0.67 | - |
cezomycin-CoA | at pH 7.5 and 30°C | Streptomyces chartreusis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not influenced by Zn2+ | Streptomyces chartreusis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces chartreusis | - |
- |
- |
Streptomyces chartreusis NRRL 3882 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and HisTrap column chromatography | Streptomyces chartreusis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O | 23% activity compared to cezomycin-CoA | Streptomyces chartreusis | CoA + acetate | - |
? | |
acetyl-CoA + H2O | 23% activity compared to cezomycin-CoA | Streptomyces chartreusis NRRL 3882 | CoA + acetate | - |
? | |
benzoyl-CoA + H2O | 16% activity compared to cezomycin-CoA | Streptomyces chartreusis | CoA + benzoate | - |
? | |
benzoyl-CoA + H2O | 16% activity compared to cezomycin-CoA | Streptomyces chartreusis NRRL 3882 | CoA + benzoate | - |
? | |
butyryl-CoA + H2O | 24% activity compared to cezomycin-CoA | Streptomyces chartreusis | CoA + butyrate | - |
? | |
cezomycin-CoA + H2O | 100% activity | Streptomyces chartreusis | CoA + cezomycin | - |
? | |
malonyl-CoA + H2O | 20% activity compared to cezomycin-CoA | Streptomyces chartreusis | CoA + malonate | - |
? | |
malonyl-CoA + H2O | 20% activity compared to cezomycin-CoA | Streptomyces chartreusis NRRL 3882 | CoA + malonate | - |
? | |
additional information | no activity with 3-S-N-acetylcysteamine. The enzyme is not involved in the release of calcimycin from the polyketide megasynthase | Streptomyces chartreusis | ? | - |
? | |
additional information | no activity with 3-S-N-acetylcysteamine. The enzyme is not involved in the release of calcimycin from the polyketide megasynthase | Streptomyces chartreusis NRRL 3882 | ? | - |
? | |
palmitoyl-CoA + H2O | 1% activity compared to cezomycin-CoA | Streptomyces chartreusis | CoA + palmitate | - |
? | |
palmitoyl-CoA + H2O | 1% activity compared to cezomycin-CoA | Streptomyces chartreusis NRRL 3882 | CoA + palmitate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27900, SDS-PAGE | Streptomyces chartreusis |
Synonyms | Comment | Organism |
---|---|---|
CalG | - |
Streptomyces chartreusis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
palmitoyl-CoA | at pH 7.5 and 30°C | Streptomyces chartreusis | |
0.015 | - |
benzoyl-CoA | at pH 7.5 and 30°C | Streptomyces chartreusis | |
0.017 | - |
malonyl-CoA | at pH 7.5 and 30°C | Streptomyces chartreusis | |
0.021 | - |
acetyl-CoA | at pH 7.5 and 30°C | Streptomyces chartreusis | |
0.021 | - |
butyryl-CoA | at pH 7.5 and 30°C | Streptomyces chartreusis | |
0.3 | - |
cezomycin-CoA | at pH 7.5 and 30°C | Streptomyces chartreusis |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme prevents the accumulation of cezomycin-CoA when intracellular nitrogen is limited | Streptomyces chartreusis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0062 | - |
palmitoyl-CoA | at pH 7.5 and 30°C | Streptomyces chartreusis | |
0.011 | - |
butyryl-CoA | at pH 7.5 and 30°C | Streptomyces chartreusis | |
0.071 | - |
benzoyl-CoA | at pH 7.5 and 30°C | Streptomyces chartreusis | |
0.09 | - |
malonyl-CoA | at pH 7.5 and 30°C | Streptomyces chartreusis | |
0.105 | - |
acetyl-CoA | at pH 7.5 and 30°C | Streptomyces chartreusis | |
0.442 | - |
cezomycin-CoA | at pH 7.5 and 30°C | Streptomyces chartreusis |