Cloned (Comment) | Organism |
---|---|
analysis carried out with a deletion mutant lacking the first 35 amino acids DELTA1-34. Expressed in Escherichia coli as a His-tagged fusion protein | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
enzyme forms homodimers in crystals. DELTA1-34 deletion mutant is crystallized to 1.45 A resolution | Homo sapiens |
enzyme forms homodimers in crystals. DELTA1-34 deletion mutant is crystallized to 1.6 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D167A | D167 is a key catalytic residue. D167A no longer displays catalytic activity | Homo sapiens |
DELTA1-37 | mutant lacking the first 37 N-terminal amino acids is excluded from mitochondria | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
palmitoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens | |
0.0029 | - |
vaccenoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens | |
0.0042 | - |
stearoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens | |
0.0042 | - |
linoleoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens | |
0.0086 | - |
myristoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens | |
0.0154 | - |
linolenoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens | |
0.019 | - |
Arachidonoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens | |
0.026 | - |
palmitoleoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Mus musculus | 5739 | - |
mitochondrion | - |
Homo sapiens | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q5T1C6 | - |
- |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonoyl-CoA + H2O | - |
Homo sapiens | CoA + arachidonate | - |
? | |
linolenoyl-CoA + H2O | - |
Homo sapiens | CoA + linolenoate | - |
? | |
linoleoyl-CoA + H2O | - |
Homo sapiens | CoA + linolate | - |
? | |
myristoyl-CoA + H2O | - |
Homo sapiens | CoA + myristic acid | - |
? | |
palmitoleoyl-CoA + H2O | - |
Homo sapiens | CoA + palmitoleoate | - |
? | |
palmitoyl-CoA + H2O | - |
Homo sapiens | CoA + palmitate | - |
? | |
stearoyl-CoA + H2O | - |
Homo sapiens | CoA + stearate | - |
? | |
vaccenoyl-CoA + H2O | - |
Homo sapiens | CoA + vaccenoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | gel filtration | Homo sapiens |
homodimer | gel filtration | Homo sapiens |
homotetramer | gel filtration, only minor fraction | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
acyl-CoA thioesterase | - |
Mus musculus |
acyl-CoA thioesterase | - |
Homo sapiens |
Them4 | - |
Homo sapiens |
Them5 | - |
Mus musculus |
Them5 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
stearoyl-CoA | pH 7.5, 37°C, mutant D167A | Homo sapiens | |
0.5 | - |
palmitoyl-CoA | pH 7.5, 37°C, mutant D167A | Homo sapiens | |
0.8 | - |
linoleoyl-CoA | pH 7.5, 37°C, mutant D167A | Homo sapiens | |
1.2 | - |
stearoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens | |
1.4 | - |
Arachidonoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens | |
1.8 | - |
vaccenoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens | |
11.5 | - |
palmitoleoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens | |
12.2 | - |
linoleoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens | |
13.3 | - |
linolenoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens | |
16.6 | - |
palmitoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens | |
19.7 | - |
myristoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | Them5 knockout mice display changes in the lipid profile of cardiolipin and its metabolites. Them5 loss results in an 2fold decrease in free fatty acids in mitochondria, with a particularly marked decrease in linoleic and linolenic acids in the knockout samples. Them5 knockout mice develop fatty liver. In addition, loss of thioesterase activity of Them5 leads to changes in mitochondrial morphology and function | Mus musculus |
malfunction | Them5-/- mice show deregulation of lipid metabolism and the development of fatty liver, exacerbated by a high-fat diet. Mitochondrial morphology is affected, and functions such as respiration and beta-oxidation are impaired | Mus musculus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
73 | - |
Arachidonoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens | |
290 | - |
stearoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens | |
430 | - |
palmitoleoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens | |
540 | - |
vaccenoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens | |
860 | - |
linolenoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens | |
2300 | - |
myristoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens | |
2900 | - |
linoleoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens | |
6900 | - |
palmitoyl-CoA | pH 7.5, 37°C, wild-type | Homo sapiens |