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Literature summary for 3.1.2.20 extracted from

  • Zhuravleva, E.; Gut, H.; Hynx, D.; Marcellin, D.; Bleck, C.K.; Genoud, C.; Cron, P.; Keusch, J.J.; Dummler, B.; Esposti, M.D.; Hemmings, B.A.
    Acyl coenzyme A thioesterase Them5/Acot15 is involved in cardiolipin remodeling and fatty liver development (2012), Mol. Cell. Biol., 32, 2685-2697.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
analysis carried out with a deletion mutant lacking the first 35 amino acids DELTA1-34. Expressed in Escherichia coli as a His-tagged fusion protein Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme forms homodimers in crystals. DELTA1-34 deletion mutant is crystallized to 1.45 A resolution Homo sapiens
enzyme forms homodimers in crystals. DELTA1-34 deletion mutant is crystallized to 1.6 A resolution Homo sapiens

Protein Variants

Protein Variants Comment Organism
D167A D167 is a key catalytic residue. D167A no longer displays catalytic activity Homo sapiens
DELTA1-37 mutant lacking the first 37 N-terminal amino acids is excluded from mitochondria Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0024
-
palmitoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
0.0029
-
vaccenoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
0.0042
-
stearoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
0.0042
-
linoleoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
0.0086
-
myristoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
0.0154
-
linolenoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
0.019
-
Arachidonoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
0.026
-
palmitoleoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Mus musculus 5739
-
mitochondrion
-
Homo sapiens 5739
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q5T1C6
-
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonoyl-CoA + H2O
-
Homo sapiens CoA + arachidonate
-
?
linolenoyl-CoA + H2O
-
Homo sapiens CoA + linolenoate
-
?
linoleoyl-CoA + H2O
-
Homo sapiens CoA + linolate
-
?
myristoyl-CoA + H2O
-
Homo sapiens CoA + myristic acid
-
?
palmitoleoyl-CoA + H2O
-
Homo sapiens CoA + palmitoleoate
-
?
palmitoyl-CoA + H2O
-
Homo sapiens CoA + palmitate
-
?
stearoyl-CoA + H2O
-
Homo sapiens CoA + stearate
-
?
vaccenoyl-CoA + H2O
-
Homo sapiens CoA + vaccenoate
-
?

Subunits

Subunits Comment Organism
dimer gel filtration Homo sapiens
homodimer gel filtration Homo sapiens
homotetramer gel filtration, only minor fraction Homo sapiens

Synonyms

Synonyms Comment Organism
acyl-CoA thioesterase
-
Mus musculus
acyl-CoA thioesterase
-
Homo sapiens
Them4
-
Homo sapiens
Them5
-
Mus musculus
Them5
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
stearoyl-CoA pH 7.5, 37°C, mutant D167A Homo sapiens
0.5
-
palmitoyl-CoA pH 7.5, 37°C, mutant D167A Homo sapiens
0.8
-
linoleoyl-CoA pH 7.5, 37°C, mutant D167A Homo sapiens
1.2
-
stearoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
1.4
-
Arachidonoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
1.8
-
vaccenoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
11.5
-
palmitoleoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
12.2
-
linoleoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
13.3
-
linolenoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
16.6
-
palmitoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
19.7
-
myristoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens

General Information

General Information Comment Organism
malfunction Them5 knockout mice display changes in the lipid profile of cardiolipin and its metabolites. Them5 loss results in an 2fold decrease in free fatty acids in mitochondria, with a particularly marked decrease in linoleic and linolenic acids in the knockout samples. Them5 knockout mice develop fatty liver. In addition, loss of thioesterase activity of Them5 leads to changes in mitochondrial morphology and function Mus musculus
malfunction Them5-/- mice show deregulation of lipid metabolism and the development of fatty liver, exacerbated by a high-fat diet. Mitochondrial morphology is affected, and functions such as respiration and beta-oxidation are impaired Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
73
-
Arachidonoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
290
-
stearoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
430
-
palmitoleoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
540
-
vaccenoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
860
-
linolenoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
2300
-
myristoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
2900
-
linoleoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens
6900
-
palmitoyl-CoA pH 7.5, 37°C, wild-type Homo sapiens