Literature summary for 3.1.2.20 extracted from

Chruszcz, M.; Zimmerman, M.D.; Wang, S.; Koclega, K.D.; Zheng, H.; Evdokimova, E.; Kudritska, M.; Cymborowski, M.; Savchenko, A.; Edwards, A.; Minor, W.
Function-Biased Choice of Additives for Optimization of Protein Crystallization: The Case of the Putative Thioesterase PA5185 from Pseudomonas aeruginosa PAO1 (2008), Cryst. Growth Des., 8, 4054-4061.

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	PA5185 contains Asp26	Pseudomonas aeruginosa PAO1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli B834(DE3)pLysS	Pseudomonas aeruginosa PAO1
native protein expressed using a modified pET-15b plasmid transformed into B834-(DE3)pLysS Escherichia coli cells	Pseudomonas aeruginosa PAO1

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals of Se-Met-substituted protein or of the native protein obtained by hanging-drop vapor diffusion, to 1.9 A resolution. Usage of additives (ATP, 4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid, 2-(N-morpholino)ethanesulfonic acid at pH 5.9, 3-(N-morpholino)propanesulfonic acid, HEPES at pH 7.0, sodium diphosphate, glucose-6-phosphate, nondetergent sulfobetaines 201 or 256, sodium CoA, lithium myristoyl-CoA, lithium benzoyl-CoA and lithium acetyl-CoA), yielding new crystal forms. The PA5185 protein crystallizes in four different forms. Crystal growth of PA5185 optimized by applying additives with chemical similarity to a fragment of a putative PA5185 substrate (CoA or its derivative). Application of function-biased additives can be used as a standard optimization protocol for producing improved diffraction, or new crystal forms, which may lead to better understanding of the biological functions of proteins like PA5185	Pseudomonas aeruginosa PAO1
hanging-drop vapor diffusion method	Pseudomonas aeruginosa PAO1

Crystallization (Comment)

Organism

crystals of Se-Met-substituted protein or of the native protein obtained by hanging-drop vapor diffusion, to 1.9 A resolution. Usage of additives (ATP, 4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid, 2-(N-morpholino)ethanesulfonic acid at pH 5.9, 3-(N-morpholino)propanesulfonic acid, HEPES at pH 7.0, sodium diphosphate, glucose-6-phosphate, nondetergent sulfobetaines 201 or 256, sodium CoA, lithium myristoyl-CoA, lithium benzoyl-CoA and lithium acetyl-CoA), yielding new crystal forms. The PA5185 protein crystallizes in four different forms. Crystal growth of PA5185 optimized by applying additives with chemical similarity to a fragment of a putative PA5185 substrate (CoA or its derivative). Application of function-biased additives can be used as a standard optimization protocol for producing improved diffraction, or new crystal forms, which may lead to better understanding of the biological functions of proteins like PA5185

Pseudomonas aeruginosa PAO1

hanging-drop vapor diffusion method

Pseudomonas aeruginosa PAO1

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa PAO1	-	-	-
Pseudomonas aeruginosa PAO1	Q9HU04	-	-

Purification (Commentary)

Purification (Comment)	Organism
immobilized metal ion affinity chromatography (Ni2+), gel filtration	Pseudomonas aeruginosa PAO1

Subunits

Subunits	Comment	Organism
homotetramer	gel filtration	Pseudomonas aeruginosa PAO1
tetramer	experimental data and theoretical predictions, gel filtration	Pseudomonas aeruginosa PAO1

Synonyms

Synonyms	Comment	Organism
PA5185	-	Pseudomonas aeruginosa PAO1
thioesterase PA5185	-	Pseudomonas aeruginosa PAO1