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Literature summary for 3.1.2.20 extracted from

  • Ofman, R.; el Mrabet, L.; Dacremont, G.; Spijer, D.; Wanders, R.J.A.
    Demonstration of dimethylnonanoyl-CoA thioesterase activity in rat liver peroxisomes followed by purification and molecular cloning of the thioesterase involved (2002), Biochem. Biophys. Res. Commun., 290, 629-634.
    View publication on PubMed
Search for more literature for 3.1.2.20

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis, expression in Escherichia coli fused to maltose-binding protein Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
 Rattus norvegicus 5777
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 x * 35000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4,8-dimethylnonanoyl-CoA + H2O Rattus norvegicus involved in pristanic acid beta-oxidation, scheme of the pathway CoA + 4,8-dimethylnonanoate
-
 ?
4,8-dimethylnonanoyl-CoA + H2O Rattus norvegicus hydrolysis allows transport of 4,8-dimethylnonanoate from peroxisomes to mitochondria for full oxidation CoA + 4,8-dimethylnonanoate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus Q8VHK0
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification of the 4,8-dimethylnonanoyl-CoA hydrolysing enzyme from liver peroxisomes Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4,8-dimethylnonanoyl-CoA + H2O
-
 Rattus norvegicus CoA + 4,8-dimethylnonanoate
-
 ?
4,8-dimethylnonanoyl-CoA + H2O involved in pristanic acid beta-oxidation, scheme of the pathway Rattus norvegicus CoA + 4,8-dimethylnonanoate
-
 ?
4,8-dimethylnonanoyl-CoA + H2O hydrolysis allows transport of 4,8-dimethylnonanoate from peroxisomes to mitochondria for full oxidation Rattus norvegicus CoA + 4,8-dimethylnonanoate
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Rattus norvegicus