General Stability | Organism |
---|---|
no dithioerythritol or other mercaptam required to maintain activity | Anas platyrhynchos |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide | 23% inhibition at 10 mM | Anas platyrhynchos | |
diethyldicarbonate | 95% inhibition at 2 mM | Anas platyrhynchos | |
diisopropylfluorophosphate | 94% inhibition at 5 mM, maximal inhibition | Anas platyrhynchos | |
p-hydroxymercuribenzoate | 99% inhibition at 0.1 mM | Anas platyrhynchos | |
PMSF | 96% inhibition at 1 mM | Anas platyrhynchos |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Anas platyrhynchos | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56300 | - |
gel filtration | Anas platyrhynchos |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + H2O | Anas platyrhynchos | - |
CoA + a carboxylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anas platyrhynchos | - |
4 isoforms of different pI and MW | - |
Anas platyrhynchos | - |
Peking or mallard duck | - |
Purification (Comment) | Organism |
---|---|
184fold | Anas platyrhynchos |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
uropygial gland | - |
Anas platyrhynchos | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
45.4 | - |
partially purified enzyme | Anas platyrhynchos |
Storage Stability | Organism |
---|---|
-20°C, purified lyophilized, at least 6 months | Anas platyrhynchos |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + H2O | - |
Anas platyrhynchos | CoA + a carboxylate | - |
? | |
acyl-CoA + H2O | broad specificity for medium-chain to long-chain acyl-CoA | Anas platyrhynchos | CoA + a carboxylate | - |
? | |
decanoyl-CoA + H2O | - |
Anas platyrhynchos | CoA + decanoate | - |
? | |
dodecanoyl-CoA + H2O | i.e. lauroyl-CoA | Anas platyrhynchos | CoA + dodecanoate | - |
? | |
additional information | substrate specificity alters with pH | Anas platyrhynchos | ? | - |
? | |
octanoyl-CoA + H2O | - |
Anas platyrhynchos | CoA + octanoate | - |
? | |
tetradecanoyl-CoA + H2O | i.e. myristoyl-CoA | Anas platyrhynchos | CoA + tetradecanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55800-59400, 4 isoforms, SDS-PAGE | Anas platyrhynchos |
Synonyms | Comment | Organism |
---|---|---|
More | cf. EC 3.1.2.19 | Anas platyrhynchos |
More | immunologically not related with the thioesterase of fatty acid synthase or the acyl fatty acid synthase thioester hydrolase | Anas platyrhynchos |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Anas platyrhynchos |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Anas platyrhynchos |
9.3 | - |
dodecanoyl-CoA as substrate | Anas platyrhynchos |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.6 | 10 | about half-maximal activity at pH 7.6 and pH 10.0, dodecanoyl-CoA as substrate | Anas platyrhynchos |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Anas platyrhynchos | pH 4.4, 4.45, 4.7, 4.9: pIs of isoenzymes | - |
4.4 |
Anas platyrhynchos | pH 4.4, 4.45, 4.7, 4.9: pIs of isoenzymes | - |
4.45 |
Anas platyrhynchos | pH 4.4, 4.45, 4.7, 4.9: pIs of isoenzymes | 4.8 | 4.7 |
Anas platyrhynchos | pH 4.4, 4.45, 4.7, 4.9: pIs of isoenzymes | - |
4.9 |