Literature summary for 3.1.2.20 extracted from

Naggert, J.; Narasimhan, M.L.; DeVeaux, L.; Cho, H.; Randhawa, Z.I.; Cronan, J.E.; Green, B.N.; Smith, S.
Cloning, sequencing, and characterization of Escherichia coli thioesterase II (1991), J. Biol. Chem., 266, 11044-11050.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene tes B, DNA sequence determination and analysis, no sequence similarity between Escherichia coli thioesterase II and the two types of mammalian thioesterases, i.e. the chain-terminating enzymes of de novo fatty acid synthesis	Escherichia coli
gene tesB, overexpression in the Escherichia coli host strain	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop method, protein solution: 10 mg/ml, 5 mM NH4OH, 2 mM DTT, over equal volume of reservoir solution: 1-2 M NaCl, 0.1 M sodium formate, pH 6.0, 2 mM N,N-dimethyl-dodecylamine oxide-LDAO, few days, X-ray structure determination and analysis	Escherichia coli

General Stability

General Stability	Organism
bovine serum albumin stabilizes	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
diethyldicarbonate	hydroxylamine reverses	Escherichia coli
IAA	-	Escherichia coli
additional information	no inhibition by iodoacetate	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Escherichia coli	5829	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
123000	-	recombinant enzyme, native PAGE	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0AGG2	gene tesB	-

Purification (Commentary)

Purification (Comment)	Organism
native wild-type: 8000fold to homogeneity	Escherichia coli
recombinant wild-type: 4fold to homogeneity	Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
93.8	-	purified recombinant enzyme	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acyl-CoA + H2O	-	Escherichia coli	CoA + a carboxylate	-	?
arachidoyl-CoA + H2O	-	Escherichia coli	CoA + arachidate	-	?
decanoyl-CoA + H2O	-	Escherichia coli	CoA + decanoate	-	?
additional information	decanoyl pantetheine is no substrate	Escherichia coli	?	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 31842-32100, recombinant enzyme, electrospray ionization mass spectroscopy and SDS-PAGE	Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Escherichia coli

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Release 2023.1 (January 2023)