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Literature summary for 3.1.2.2 extracted from
- Identification of a type III thioesterase reveals the function of an operon crucial for Mtb virulence (2007), Chem. Biol., 14, 543-551.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Mycobacterium tuberculosis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging-drop vapor-diffusion method, crystal structure to 2.3 A resolution. FcoT crystals, produced in the presence of the substrate dodecenoyl-CoA, are cubic and belong to space group I2(1)3, with one molecule in the asymmetric unit | Mycobacterium tuberculosis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E77Q | mutation decreased the enzyme activity to less than 10fold of wild-type | Mycobacterium tuberculosis |
| H72A | mutation decreased the enzyme activity to less than 10fold of wild-type | Mycobacterium tuberculosis |
| N74A | mutation abolishes the activity of the enzyme | Mycobacterium tuberculosis |
| N83A | mutation decreased the enzyme activity to less than 10fold of wild-type | Mycobacterium tuberculosis |
| Y33F | mutation abolishes the activity of the enzyme | Mycobacterium tuberculosis |
| Y66F | mutation abolishes the activity of the enzyme | Mycobacterium tuberculosis |
| Y87F | mutation decreased the enzyme activity to less than 10fold of wild-type | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01187 | - |
palmitoyl-CoA | pH 7.5 | Mycobacterium tuberculosis |  |
| 0.01913 | - |
dodecanoyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
| 0.03852 | - |
lauroyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
| 0.06318 | - |
hexanoyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
| 0.08735 | - |
crotonyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
| 0.1028 | - |
stearoyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
| 0.272 | - |
benzoyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | P9WM67 | - |
- |
| Mycobacterium tuberculosis H37Rv | P9WM67 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoyl-CoA + H2O | - |
Mycobacterium tuberculosis | CoA + benzoate | - |
? | |
| crotonyl-CoA + H2O | - |
Mycobacterium tuberculosis | CoA + crotonate | - |
? | |
| crotonyl-CoA + H2O | - |
Mycobacterium tuberculosis H37Rv | CoA + crotonate | - |
? | |
| dodecanoyl-CoA + H2O | - |
Mycobacterium tuberculosis | CoA + dodecanoate | - |
? | |
| dodecanoyl-CoA + H2O | - |
Mycobacterium tuberculosis H37Rv | CoA + dodecanoate | - |
? | |
| hexanoyl-CoA + H2O | - |
Mycobacterium tuberculosis | CoA + hexanoate | - |
? | |
| hexanoyl-CoA + H2O | - |
Mycobacterium tuberculosis H37Rv | CoA + hexanoate | - |
? | |
| lauroyl-CoA + H2O | - |
Mycobacterium tuberculosis | CoA + laurate | - |
? | |
| palmitoyl-CoA + H2O | - |
Mycobacterium tuberculosis | CoA + palmitate | - |
? | |
| palmitoyl-CoA + H2O | - |
Mycobacterium tuberculosis H37Rv | CoA + palmitate | - |
? | |
| stearoyl-CoA + H2O | - |
Mycobacterium tuberculosis | CoA + stearate | - |
? | |
| stearoyl-CoA + H2O | - |
Mycobacterium tuberculosis H37Rv | CoA + stearate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FcoT | - |
Mycobacterium tuberculosis |
| long-chain fatty acyl-CoA thioesterase | - |
Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
benzoyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
| 0.0078 | - |
crotonyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
| 0.01 | - |
dodecanoyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
| 0.012 | - |
hexanoyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
| 0.015 | - |
lauroyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
| 0.019 | - |
stearoyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
| 0.037 | - |
palmitoyl-CoA | pH 7.5 | Mycobacterium tuberculosis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | below pH 7 there is no detectable activity. In the range of pH 78.5, the activity shows a linear phase and reaches a plateau at pH 8.5 | Mycobacterium tuberculosis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | below pH 7 there is no detectable activity. In the range of pH 78.5, the activity shows a linear phase and reaches a plateau at pH 8.5 | Mycobacterium tuberculosis |
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