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Literature summary for 3.1.2.2 extracted from

  • Lee, L.C.; Lee, Y.L.; Leu, R.J.; Shaw, J.F.
    Functional role of catalytic triad and oxyanion hole-forming residues on enzyme activity of Escherichia coli thioesterase I/protease I/phospholipase L1 (2006), Biochem. J., 397, 69-76.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.2.2

Protein Variants

Protein Variants Comment Organism
D154A kcat/KM for p-nitrophenyl butyrate is 18% of wild-type value. kcat/KM for lauroyl-CoA is 11% of wild-type value. kcat/KM for N-carbobenzoxy-L-tyrosine p-nitrophenyl ester is 29% of wild-type value Escherichia coli
G44A kcat/KM for p-nitrophenyl butyrate is 16% of wild-type value. kcat/KM for lauroyl-CoA is 15% of wild-type value. kcat/KM for N-carbobenzoxy-L-tyrosine p-nitrophenyl ester is 25% of wild-type value Escherichia coli
H157A kcat/KM for p-nitrophenyl butyrate is 0.09% of wild-type value Escherichia coli
N73A kcat/KM for p-nitrophenyl butyrate is 41% of wild-type value. kcat/KM for lauroyl-CoA is 19% of wild-type value. kcat/KM for N-carbobenzoxy-L-tyrosine p-nitrophenyl ester is 35% of wild-type value Escherichia coli
S10A kcat/KM for p-nitrophenyl butyrate is 0.57% of wild-type value. kcat/KM for lauroyl-CoA is 0.6% of wild-type value. kcat/KM for N-carbobenzoxy-L-tyrosine p-nitrophenyl ester is 0.06% of wild-type value Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, mutant enzyme D154A Escherichia coli
0.044
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, mutant enzyme N73A Escherichia coli
0.059
-
 lauroyl-CoA 37°C, pH 7, mutant enzyme D154A Escherichia coli
0.06
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, mutant enzyme G44A Escherichia coli
0.061
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, mutant enzyme, S10A Escherichia coli
0.075
-
 lauroyl-CoA 37°C, pH 7, mutant enzyme G44A Escherichia coli
0.085
-
 lauroyl-CoA 37°C, pH 7, mutant enzyme N73A Escherichia coli
0.127
-
 lauroyl-CoA 37°C, pH 7, mutant enzyme, S10A Escherichia coli
0.146
-
 lauroyl-CoA 37°C, pH 7, wild-type enzyme Escherichia coli
0.174
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, wild-type enzyme Escherichia coli
0.61
-
 p-nitrophenyl butyrate 37°C, pH 7, mutant enzyme H157A Escherichia coli
0.87
-
 p-nitrophenyl butyrate 37°C, pH 7, wild-type enzyme Escherichia coli
1.1
-
 p-nitrophenyl butyrate 37°C, pH 7, mutant enzyme G44A Escherichia coli
1.55
-
 p-nitrophenyl butyrate 37°C, pH 7, mutant enzyme S10A Escherichia coli
1.66
-
 p-nitrophenyl butyrate 37°C, pH 7, mutant enzyme N73A Escherichia coli
1.88
-
 p-nitrophenyl butyrate 37°C, pH 7, mutant enzyme D154A Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0ADA1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lauroyl-CoA + H2O
-
 Escherichia coli CoA + laurate
-
 ?
N-carbobenzoxy-L-tyrosine p-nitrophenyl ester + H2O
-
 Escherichia coli N-carbobenzoxy-L-tyrosine + p-nitrophenol
-
 ?
p-nitrophenyl butyrate + H2O
-
 Escherichia coli p-nitrophenol + butyrate
-
 ?

Synonyms

Synonyms Comment Organism
TAP
-
 Escherichia coli
thioesterase I multifunctional enzyme with thioesterase, esterase, arylesterase, protease and lysophospholipase activities Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, mutant enzyme D154A Escherichia coli
0.0093
-
 p-nitrophenyl butyrate 37°C, pH 7, mutant enzyme H157A Escherichia coli
0.02
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, mutant enzyme, S10A Escherichia coli
0.031 0.51 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, mutant enzyme G44A Escherichia coli
0.052 2.1 lauroyl-CoA 37°C, pH 7, wild-type enzyme Escherichia coli
0.057
-
 lauroyl-CoA 37°C, pH 7, mutant enzyme, S10A Escherichia coli
0.16
-
 p-nitrophenyl butyrate 37°C, pH 7, mutant enzyme S10A Escherichia coli
0.41
-
 p-nitrophenyl butyrate 37°C, pH 7, mutant enzyme N73A Escherichia coli
0.45
-
 lauroyl-CoA 37°C, pH 7, mutant enzyme D154A Escherichia coli
0.55
-
 p-nitrophenyl butyrate 37°C, pH 7, wild-type enzyme Escherichia coli
0.77
-
 lauroyl-CoA 37°C, pH 7, mutant enzyme G44A Escherichia coli
1.14
-
 lauroyl-CoA 37°C, pH 7, mutant enzyme N73A Escherichia coli
3.16
-
 p-nitrophenyl butyrate 37°C, pH 7, mutant enzyme G44A Escherichia coli
5.98
-
 p-nitrophenyl butyrate 37°C, pH 7, mutant enzyme D154A Escherichia coli
6.44
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, mutant enzyme D154A Escherichia coli
7.71
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, mutant enzyme G44A Escherichia coli
7.81
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, mutant enzyme N73A Escherichia coli
10.13
-
 lauroyl-CoA 37°C, pH 7, wild-type enzyme Escherichia coli
11.99
-
 p-nitrophenyl butyrate 37°C, pH 7, mutant enzyme N73A Escherichia coli
15.29
-
 p-nitrophenyl butyrate 37°C, pH 7, wild-type enzyme Escherichia coli
23.8
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, wild-type enzyme Escherichia coli
88.99
-
 N-carbobenzoxy-L-tyrosine p-nitrophenyl ester 37°C, pH 7, wild-type enzyme Escherichia coli