Any feedback?
Literature summary for 3.1.2.2 extracted from
- Two esterases released from Mycobacterium smegmatis for the hydrolysis of long chain acyl-CoAs and Tween (1981), J. Biochem., 90, 1661-1669.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Mycolicibacterium smegmatis |  |
| diisopropylfluorophosphate | 1 mM inhibits activity completely | Mycolicibacterium smegmatis | |
| Mg2+ | - |
Mycolicibacterium smegmatis | |
| papain | - |
Mycolicibacterium smegmatis | |
| SDS | - |
Mycolicibacterium smegmatis | |
| Triton X-100 | - |
Mycolicibacterium smegmatis | |
| Trypsin | - |
Mycolicibacterium smegmatis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0004 | - |
eicosanoyl-CoA | - |
Mycolicibacterium smegmatis | |
| 0.005 | - |
oleoyl-CoA | - |
Mycolicibacterium smegmatis | |
| 0.0062 | - |
hexadecanoyl-CoA | - |
Mycolicibacterium smegmatis | |
| 0.0066 | - |
tetradecanoyl-CoA | - |
Mycolicibacterium smegmatis | |
| 0.0077 | - |
dodecanoyl-CoA | - |
Mycolicibacterium smegmatis | |
| 0.047 | - |
hexadecanoyl-CoA | - |
Mycolicibacterium smegmatis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
gel filtration | Mycolicibacterium smegmatis |
| 42000 | - |
x * 42000, SDS-PAGE | Mycolicibacterium smegmatis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| palmitoyl-CoA + H2O | Mycolicibacterium smegmatis | - |
CoA + palmitate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycolicibacterium smegmatis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mycolicibacterium smegmatis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 219 | - |
- |
Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + H2O | long-chain fatty-acyl-CoA | Mycolicibacterium smegmatis | CoA + a carboxylate | - |
? | |
| arachidonoyl-CoA + H2O | - |
Mycolicibacterium smegmatis | CoA + arachidonate | - |
? | |
| dodecanoyl-CoA + H2O | i.e. lauroyl-CoA | Mycolicibacterium smegmatis | CoA + dodecanoate | - |
? | |
| eicosanoyl-CoA + H2O | - |
Mycolicibacterium smegmatis | CoA + eicosanoate | - |
? | |
| hexadecanoyl-CoA + H2O | i.e. palmitoyl-CoA | Mycolicibacterium smegmatis | CoA + hexadecanoate | - |
? | |
| monoolein + H2O | - |
Mycolicibacterium smegmatis | oleate + glycerol | - |
? | |
| octadecanoyl-CoA + H2O | i.e. stearoyl-CoA | Mycolicibacterium smegmatis | CoA + octadecanoate | - |
? | |
| oleoyl-CoA + H2O | - |
Mycolicibacterium smegmatis | CoA + oleate | - |
? | |
| palmitoyl-CoA + H2O | - |
Mycolicibacterium smegmatis | CoA + palmitate | - |
? | |
| tetradecanoyl-CoA + H2O | i.e. myristoyl-CoA | Mycolicibacterium smegmatis | CoA + tetradecanoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, SDS-PAGE | Mycolicibacterium smegmatis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Mycolicibacterium smegmatis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | - |
Mycolicibacterium smegmatis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Mycolicibacterium smegmatis |
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