KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
(S)-methylmalonyl-CoA | pH 6.0, 30°C | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
- |
Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.41 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme stable for at least 1 month | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-methylmalonyl-CoA + H2O | not active on the L-isomer | Rattus norvegicus | methylmalonate + CoA | - |
? | |
malonyl-CoA + H2O | 16% of the activity with (S)-methylmalonyl-CoA | Rattus norvegicus | malonate + CoA | - |
? | |
additional information | acetyl-CoA 1% of the activity with (S)-methylmalonyl-CoA , succinyl-CoA and palmitoyl-CoA hydrolyzed at less than 1% of the activity with (S)-methylmalonyl-CoA | Rattus norvegicus | ? | - |
? | |
propionyl-CoA + H2O | 3% of the activity with (S)-methylmalonyl-CoA | Rattus norvegicus | propionate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000, SDS-PAGE, in absence and presence of 1% 2-mercaptoethanol | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
D-methylmalonyl-CoA hydrolase | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23.3 | - |
(S)-methylmalonyl-CoA | pH 6.0, 30°C | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8.5 | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactors identified | Rattus norvegicus |