BRENDA - Enzyme Database
show all sequences of 3.1.2.17

Recognition, isolation, and characterization of rat liver D-methylmalonyl coenzyme A hydrolase

Kovachy, R.J.; Copley, S.D.; Allen, R.H.; J. Biol. Chem. 258, 11415-11421 (1983)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
acetyl-CoA
competitive inhibition
Rattus norvegicus
butyryl-CoA
competitive
Rattus norvegicus
CoA
competitive inhibition
Rattus norvegicus
decanoyl-CoA
noncompetitive inhibition
Rattus norvegicus
glutaryl-CoA
competitive inhibition
Rattus norvegicus
heptanoyl-CoA
competitive inhibition
Rattus norvegicus
hexanoyl-CoA
competitive inhibition
Rattus norvegicus
malonyl-CoA
competitive inhibition
Rattus norvegicus
nonanoyl-CoA
noncompetitive inhibition
Rattus norvegicus
octanoyl-CoA
noncompetitive inhibition
Rattus norvegicus
palmitoyl-CoA
noncompetitive inhibition
Rattus norvegicus
pentanoyl-CoA
competitive inhibition
Rattus norvegicus
propionyl-CoA
competitive inhibition
Rattus norvegicus
succinyl-CoA
competitive inhibition
Rattus norvegicus
tridecanoyl-CoA
noncompetitive inhibition
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.7
-
malonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
0.7
-
(S)-methylmalonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
1.4
-
DL-methylmalonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
1.9
-
propionyl-CoA
pH 6.0, 30°C
Rattus norvegicus
14
-
acetyl-CoA
pH 6.0, 30°C
Rattus norvegicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-methylmalonyl-CoA + H2O
Rattus norvegicus
-
methylmalonate + CoA
-
Rattus norvegicus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
partially
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.41
-
-
Rattus norvegicus
Storage Stability
Storage Stability
Organism
-20°C, purified enzyme stable for at least 1 month
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-methylmalonyl-CoA + H2O
-
643853
Rattus norvegicus
methylmalonate + CoA
-
643853
Rattus norvegicus
?
(S)-methylmalonyl-CoA + H2O
not active on the L-isomer
643853
Rattus norvegicus
methylmalonate + CoA
-
643853
Rattus norvegicus
?
malonyl-CoA + H2O
16% of the activity with (S)-methylmalonyl-CoA
643853
Rattus norvegicus
malonate + CoA
-
643853
Rattus norvegicus
?
additional information
acetyl-CoA 1% of the activity with (S)-methylmalonyl-CoA , succinyl-CoA and palmitoyl-CoA hydrolyzed at less than 1% of the activity with (S)-methylmalonyl-CoA
643853
Rattus norvegicus
?
-
-
-
-
propionyl-CoA + H2O
3% of the activity with (S)-methylmalonyl-CoA
643853
Rattus norvegicus
propionate + CoA
-
643853
Rattus norvegicus
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 35000, SDS-PAGE, in absence and presence of 1% 2-mercaptoethanol
Rattus norvegicus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
23.3
-
(S)-methylmalonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
50% activity at pH 5.3 and pH 7.1
Rattus norvegicus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.01
-
palmitoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
0.04
-
tridecanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
0.05
-
decanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
0.2
-
nonanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
0.5
-
octanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
0.6
-
malonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
1.4
-
CoA
pH 6.0, 30°C
Rattus norvegicus
1.5
-
heptanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
1.5
-
succinyl-CoA
pH 6.0, 30°C
Rattus norvegicus
1.7
-
hexanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
2.6
-
butyryl-CoA
pH 6.0, 30°C
Rattus norvegicus
2.6
-
valeryl-CoA
pH 6.0, 30°C
Rattus norvegicus
3
-
propionyl-CoA
pH 6.0, 30°C
Rattus norvegicus
4.1
-
acetyl-CoA
pH 6.0, 30°C
Rattus norvegicus
4.9
-
glutaryl-CoA
pH 6.0, 30°C
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
acetyl-CoA
competitive inhibition
Rattus norvegicus
butyryl-CoA
competitive
Rattus norvegicus
CoA
competitive inhibition
Rattus norvegicus
decanoyl-CoA
noncompetitive inhibition
Rattus norvegicus
glutaryl-CoA
competitive inhibition
Rattus norvegicus
heptanoyl-CoA
competitive inhibition
Rattus norvegicus
hexanoyl-CoA
competitive inhibition
Rattus norvegicus
malonyl-CoA
competitive inhibition
Rattus norvegicus
nonanoyl-CoA
noncompetitive inhibition
Rattus norvegicus
octanoyl-CoA
noncompetitive inhibition
Rattus norvegicus
palmitoyl-CoA
noncompetitive inhibition
Rattus norvegicus
pentanoyl-CoA
competitive inhibition
Rattus norvegicus
propionyl-CoA
competitive inhibition
Rattus norvegicus
succinyl-CoA
competitive inhibition
Rattus norvegicus
tridecanoyl-CoA
noncompetitive inhibition
Rattus norvegicus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.01
-
palmitoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
0.04
-
tridecanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
0.05
-
decanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
0.2
-
nonanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
0.5
-
octanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
0.6
-
malonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
1.4
-
CoA
pH 6.0, 30°C
Rattus norvegicus
1.5
-
heptanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
1.5
-
succinyl-CoA
pH 6.0, 30°C
Rattus norvegicus
1.7
-
hexanoyl-CoA
pH 6.0, 30°C
Rattus norvegicus
2.6
-
butyryl-CoA
pH 6.0, 30°C
Rattus norvegicus
2.6
-
valeryl-CoA
pH 6.0, 30°C
Rattus norvegicus
3
-
propionyl-CoA
pH 6.0, 30°C
Rattus norvegicus
4.1
-
acetyl-CoA
pH 6.0, 30°C
Rattus norvegicus
4.9
-
glutaryl-CoA
pH 6.0, 30°C
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.7
-
malonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
0.7
-
(S)-methylmalonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
1.4
-
DL-methylmalonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
1.9
-
propionyl-CoA
pH 6.0, 30°C
Rattus norvegicus
14
-
acetyl-CoA
pH 6.0, 30°C
Rattus norvegicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
gel filtration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-methylmalonyl-CoA + H2O
Rattus norvegicus
-
methylmalonate + CoA
-
Rattus norvegicus
?
Purification (Commentary) (protein specific)
Commentary
Organism
partially
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.41
-
-
Rattus norvegicus
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, purified enzyme stable for at least 1 month
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-methylmalonyl-CoA + H2O
-
643853
Rattus norvegicus
methylmalonate + CoA
-
643853
Rattus norvegicus
?
(S)-methylmalonyl-CoA + H2O
not active on the L-isomer
643853
Rattus norvegicus
methylmalonate + CoA
-
643853
Rattus norvegicus
?
malonyl-CoA + H2O
16% of the activity with (S)-methylmalonyl-CoA
643853
Rattus norvegicus
malonate + CoA
-
643853
Rattus norvegicus
?
additional information
acetyl-CoA 1% of the activity with (S)-methylmalonyl-CoA , succinyl-CoA and palmitoyl-CoA hydrolyzed at less than 1% of the activity with (S)-methylmalonyl-CoA
643853
Rattus norvegicus
?
-
-
-
-
propionyl-CoA + H2O
3% of the activity with (S)-methylmalonyl-CoA
643853
Rattus norvegicus
propionate + CoA
-
643853
Rattus norvegicus
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 35000, SDS-PAGE, in absence and presence of 1% 2-mercaptoethanol
Rattus norvegicus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
23.3
-
(S)-methylmalonyl-CoA
pH 6.0, 30°C
Rattus norvegicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
50% activity at pH 5.3 and pH 7.1
Rattus norvegicus
Other publictions for EC 3.1.2.17
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
643854
Kovachy
D-Methylmalonyl-CoA hydrolase ...
Rattus norvegicus
Methods Enzymol.
166
393-400
1988
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
1
-
-
1
1
1
4
1
-
-
-
1
-
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
1
1
1
4
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
643853
Kovachy
Recognition, isolation, and ch ...
Rattus norvegicus
J. Biol. Chem.
258
11415-11421
1983
-
-
-
-
-
-
15
5
-
-
1
1
-
1
-
-
1
-
-
1
1
1
5
1
-
-
-
1
1
-
-
-
15
-
-
-
-
-
-
-
-
-
-
15
15
5
-
-
1
1
-
-
-
1
-
1
1
1
5
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-