BRENDA - Enzyme Database
show all sequences of 3.1.2.16

Substrate specificity of citrate lyase deacetylase of Rhodopseudomonas gelatinosa and Rhodopseudomonas palustris

Giffhorn, F.; Zimmermann, T.; Kuhn, A.; J. Bacteriol. 147, 463-470 (1981)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
antibodies against the large subunit of citrate lyase inhibits the deacetylase, antibodies against the middle and small subunits does not
Rhodopseudomonas palustris
additional information
-
Rubrivivax gelatinosus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
[citrate (pro-3S)-lyase](acetyl form) + H2O
Rubrivivax gelatinosus
-
[citrate (pro-3S)-lyase](thiol form) + acetate
-
Rubrivivax gelatinosus
?
[citrate (pro-3S)-lyase](acetyl form) + H2O
Rhodopseudomonas palustris
-
[citrate (pro-3S)-lyase](thiol form) + acetate
-
Rhodopseudomonas palustris
?
Organism
Organism
UniProt
Commentary
Textmining
no activity in Clostridium sphenoides
-
-
-
Rhodopseudomonas palustris
-
-
-
Rubrivivax gelatinosus
-
DSM 149
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Rubrivivax gelatinosus
partially
Rhodopseudomonas palustris
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
purified citrate lyases from Enterobacter aerogenes, Streptococcus diacetilactis and Clostridium sphenoides are no substrates
643852
Rhodopseudomonas palustris
?
-
-
-
-
[citrate (pro-3S)-lyase](acetyl form) + H2O
-
643852
Rubrivivax gelatinosus
[citrate (pro-3S)-lyase](thiol form) + acetate
-
643852
Rubrivivax gelatinosus
?
[citrate (pro-3S)-lyase](acetyl form) + H2O
-
643852
Rhodopseudomonas palustris
[citrate (pro-3S)-lyase](thiol form) + acetate
-
643852
Rhodopseudomonas palustris
?
Synonyms
Synonyms
Commentary
Organism
acetyl-S-(acyl-carrier protein) enzyme thioester hydrolase (acetate)
-
Rhodopseudomonas palustris
acetyl-S-(acyl-carrier protein) enzyme thioester hydrolase (acetate)
-
Rubrivivax gelatinosus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
antibodies against the large subunit of citrate lyase inhibits the deacetylase, antibodies against the middle and small subunits does not
Rhodopseudomonas palustris
additional information
-
Rubrivivax gelatinosus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
[citrate (pro-3S)-lyase](acetyl form) + H2O
Rubrivivax gelatinosus
-
[citrate (pro-3S)-lyase](thiol form) + acetate
-
Rubrivivax gelatinosus
?
[citrate (pro-3S)-lyase](acetyl form) + H2O
Rhodopseudomonas palustris
-
[citrate (pro-3S)-lyase](thiol form) + acetate
-
Rhodopseudomonas palustris
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rubrivivax gelatinosus
partially
Rhodopseudomonas palustris
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
purified citrate lyases from Enterobacter aerogenes, Streptococcus diacetilactis and Clostridium sphenoides are no substrates
643852
Rhodopseudomonas palustris
?
-
-
-
-
[citrate (pro-3S)-lyase](acetyl form) + H2O
-
643852
Rubrivivax gelatinosus
[citrate (pro-3S)-lyase](thiol form) + acetate
-
643852
Rubrivivax gelatinosus
?
[citrate (pro-3S)-lyase](acetyl form) + H2O
-
643852
Rhodopseudomonas palustris
[citrate (pro-3S)-lyase](thiol form) + acetate
-
643852
Rhodopseudomonas palustris
?
Other publictions for EC 3.1.2.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
643852
Giffhorn
Substrate specificity of citra ...
no activity in Clostridium sphenoides, Rhodopseudomonas palustris, Rubrivivax gelatinosus
J. Bacteriol.
147
463-470
1981
-
-
-
-
-
-
2
-
-
-
-
2
-
9
-
-
2
-
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643851
Giffhorn
Citrate lyase deacetylase of R ...
no activity in Enterobacter aerogenes, no activity in Escherichia coli, no activity in Streptococcus diacetilactis, Rubrivivax gelatinosus
Eur. J. Biochem.
111
461-471
1980
-
-
-
-
-
1
5
-
-
-
2
1
-
6
-
-
1
-
-
-
1
2
2
1
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
5
1
-
-
-
2
1
-
-
-
1
-
-
1
2
2
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
643850
Giffhorn
Inactivation of citrate lyase ...
no activity in Enterobacter aerogenes, no activity in Streptococcus diacetilactis, Rubrivivax gelatinosus
J. Bacteriol.
124
1052-1061
1975
1
-
-
-
-
-
5
1
-
-
-
1
-
8
-
-
1
-
-
-
-
1
2
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
5
-
1
-
-
-
1
-
-
-
1
-
-
-
1
2
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-