General Stability | Organism |
---|---|
loses activity upon repeated freezing and thawing | Rubrivivax gelatinosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-glutamyl-glutamate | 10 mM, 70% inhibition | Rubrivivax gelatinosus | |
D-glutamate | 10 mM, 54% inhibition | Rubrivivax gelatinosus | |
gamma-glutamyl-glutamate | 10 mM, 70% inhibition | Rubrivivax gelatinosus | |
L-aspartate | 10 mM, 60% inhibition | Rubrivivax gelatinosus | |
L-glutamate | linear mixed type of inhibition | Rubrivivax gelatinosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
7300 | - |
2 * 7300, SDS-PAGE | Rubrivivax gelatinosus |
14300 | - |
gel filtration | Rubrivivax gelatinosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[citrate (pro-3S)-lyase](acetyl form) + H2O | Rubrivivax gelatinosus | regulates the activity of citrate layse by covalent modification | [citrate (pro-3S)-lyase](thiol form) + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
no activity in Enterobacter aerogenes | - |
- |
- |
no activity in Escherichia coli | - |
- |
- |
no activity in Streptococcus diacetilactis | - |
- |
- |
Rubrivivax gelatinosus | - |
DSM 149 | - |
Purification (Comment) | Organism |
---|---|
- |
Rubrivivax gelatinosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity 1000 U/mg | Rubrivivax gelatinosus |
additional information | - |
specific activity 15.00 U/mg | Rubrivivax gelatinosus |
Storage Stability | Organism |
---|---|
-20°C, partly purified enzyme retains full activity for at least 1 year | Rubrivivax gelatinosus |
-20°C, purified enzyme loses 20% of activity within 1 year during storage | Rubrivivax gelatinosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[citrate (pro-3S)-lyase](acetyl form) + H2O | - |
Rubrivivax gelatinosus | [citrate (pro-3S)-lyase](thiol form) + acetate | - |
? | |
[citrate (pro-3S)-lyase](acetyl form) + H2O | regulates the activity of citrate layse by covalent modification | Rubrivivax gelatinosus | [citrate (pro-3S)-lyase](thiol form) + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 7300, SDS-PAGE | Rubrivivax gelatinosus |
Synonyms | Comment | Organism |
---|---|---|
acetyl-S-(acyl-carrier protein) enzyme thioester hydrolase (acetate) | - |
Rubrivivax gelatinosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life 20 min, effectively protected against heat inactivation by 100 mM L-glutamate, half-life 30 min | Rubrivivax gelatinosus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
L-glutamate | pH 7.5, 30°C | Rubrivivax gelatinosus |