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show all sequences of 3.1.2.16

Inactivation of citrate lyase from Rhodopseudomonas gelatinosa by a specific deacetylase and inhibition of this inactivation by L-(+)-glutamate

Giffhorn, F.; Gottschalk, G.; J. Bacteriol. 124, 1052-1061 (1975)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
citrate
induced by
Rubrivivax gelatinosus
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
0.01 M, 40% inhibition
Rubrivivax gelatinosus
L-arginine
0.01 M, 47% inhibition
Rubrivivax gelatinosus
L-aspartate
-
Rubrivivax gelatinosus
L-glutamate
-
Rubrivivax gelatinosus
additional information
D-glutamate, 2-oxoglutarate, L-alpha-hydroxyglutarate and L-proline are less effective inhibitors, iodoacetate, iodoacetamide or N-ethylmaleimide does not interfere with deacetylase activity
Rubrivivax gelatinosus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0001
-
[citrate (pro-3S)-lyase](acetyl form)
pH 7.2, 30C
Rubrivivax gelatinosus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
[citrate (pro-3S)-lyase](acetyl form) + H2O
Rubrivivax gelatinosus
-
[citrate (pro-3S)-lyase](thiol form) + acetate
-
Rubrivivax gelatinosus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
no activity in Enterobacter aerogenes
-
-
-
no activity in Streptococcus diacetilactis
-
-
-
Rubrivivax gelatinosus
-
DSM 149
-
Purification (Commentary)
Commentary
Organism
-
Rubrivivax gelatinosus
Storage Stability
Storage Stability
Organism
-15C, purified enzyme stable for at least 6 months
Rubrivivax gelatinosus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
acetyl-CoA, acetoacetyl-CoA, S-acetyl-N-acetylcyseamine and purified citrate lyases from Enterobacter aerogenes and Streptococcus diacetilactis are no substrates, reaction with butyryl-S-citrate lyase, propionyl-S-citrate lyaseand acetyl-S-citrate lyase only slightly faster than spontaneous hydrolysis
643850
Rubrivivax gelatinosus
?
-
-
-
-
[citrate (pro-3S)-lyase](acetyl form) + H2O
-
643850
Rubrivivax gelatinosus
[citrate (pro-3S)-lyase](thiol form) + acetate
-
643850
Rubrivivax gelatinosus
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.1
-
-
Rubrivivax gelatinosus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7.2
8.1
because of the instability of the substrate acetyl-S-citrate lyase at acidic and alkaline pH-values, all assays are carried out at pH 7.2, where the spontanous hydrolysis of the substrate is negligible and the enzyme shows 70% of the activity at pH 8.1
Rubrivivax gelatinosus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
citrate
induced by
Rubrivivax gelatinosus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
0.01 M, 40% inhibition
Rubrivivax gelatinosus
L-arginine
0.01 M, 47% inhibition
Rubrivivax gelatinosus
L-aspartate
-
Rubrivivax gelatinosus
L-glutamate
-
Rubrivivax gelatinosus
additional information
D-glutamate, 2-oxoglutarate, L-alpha-hydroxyglutarate and L-proline are less effective inhibitors, iodoacetate, iodoacetamide or N-ethylmaleimide does not interfere with deacetylase activity
Rubrivivax gelatinosus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0001
-
[citrate (pro-3S)-lyase](acetyl form)
pH 7.2, 30C
Rubrivivax gelatinosus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
[citrate (pro-3S)-lyase](acetyl form) + H2O
Rubrivivax gelatinosus
-
[citrate (pro-3S)-lyase](thiol form) + acetate
-
Rubrivivax gelatinosus
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rubrivivax gelatinosus
Storage Stability (protein specific)
Storage Stability
Organism
-15C, purified enzyme stable for at least 6 months
Rubrivivax gelatinosus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
acetyl-CoA, acetoacetyl-CoA, S-acetyl-N-acetylcyseamine and purified citrate lyases from Enterobacter aerogenes and Streptococcus diacetilactis are no substrates, reaction with butyryl-S-citrate lyase, propionyl-S-citrate lyaseand acetyl-S-citrate lyase only slightly faster than spontaneous hydrolysis
643850
Rubrivivax gelatinosus
?
-
-
-
-
[citrate (pro-3S)-lyase](acetyl form) + H2O
-
643850
Rubrivivax gelatinosus
[citrate (pro-3S)-lyase](thiol form) + acetate
-
643850
Rubrivivax gelatinosus
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.1
-
-
Rubrivivax gelatinosus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7.2
8.1
because of the instability of the substrate acetyl-S-citrate lyase at acidic and alkaline pH-values, all assays are carried out at pH 7.2, where the spontanous hydrolysis of the substrate is negligible and the enzyme shows 70% of the activity at pH 8.1
Rubrivivax gelatinosus
Other publictions for EC 3.1.2.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
643852
Giffhorn
Substrate specificity of citra ...
no activity in Clostridium sphenoides, Rhodopseudomonas palustris, Rubrivivax gelatinosus
J. Bacteriol.
147
463-470
1981
-
-
-
-
-
-
2
-
-
-
-
2
-
9
-
-
2
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643851
Giffhorn
Citrate lyase deacetylase of R ...
no activity in Enterobacter aerogenes, no activity in Escherichia coli, no activity in Streptococcus diacetilactis, Rubrivivax gelatinosus
Eur. J. Biochem.
111
461-471
1980
-
-
-
-
-
1
5
-
-
-
2
1
-
6
-
-
1
-
-
-
1
2
2
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
5
1
-
-
-
2
1
-
-
-
1
-
-
1
2
2
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
643850
Giffhorn
Inactivation of citrate lyase ...
no activity in Enterobacter aerogenes, no activity in Streptococcus diacetilactis, Rubrivivax gelatinosus
J. Bacteriol.
124
1052-1061
1975
1
-
-
-
-
-
5
1
-
-
-
1
-
8
-
-
1
-
-
-
-
1
2
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
5
-
1
-
-
-
1
-
-
-
1
-
-
-
1
2
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-