BRENDA - Enzyme Database
show all sequences of 3.1.2.16

Citrate lyase deacetylase of Rhodopseudomonas gelatinosa. Isolation of the enzyme and studies on the inhibition by L-glutamate

Giffhorn, F.; Rode, H.; Kuhn, A.; Gottschalk, G.; Eur. J. Biochem. 111, 461-471 (1980)

Data extracted from this reference:

General Stability
General Stability
Organism
loses activity upon repeated freezing and thawing
Rubrivivax gelatinosus
Inhibitors
Inhibitors
Commentary
Organism
Structure
alpha-glutamyl-glutamate
10 mM, 70% inhibition
Rubrivivax gelatinosus
D-glutamate
10 mM, 54% inhibition
Rubrivivax gelatinosus
gamma-glutamyl-glutamate
10 mM, 70% inhibition
Rubrivivax gelatinosus
L-aspartate
10 mM, 60% inhibition
Rubrivivax gelatinosus
L-glutamate
linear mixed type of inhibition
Rubrivivax gelatinosus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
7300
-
2 * 7300, SDS-PAGE
Rubrivivax gelatinosus
14300
-
gel filtration
Rubrivivax gelatinosus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
[citrate (pro-3S)-lyase](acetyl form) + H2O
Rubrivivax gelatinosus
regulates the activity of citrate layse by covalent modification
[citrate (pro-3S)-lyase](thiol form) + acetate
-
Rubrivivax gelatinosus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
no activity in Enterobacter aerogenes
-
-
-
no activity in Escherichia coli
-
-
-
no activity in Streptococcus diacetilactis
-
-
-
Rubrivivax gelatinosus
-
DSM 149
-
Purification (Commentary)
Commentary
Organism
-
Rubrivivax gelatinosus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
specific activity 1000 U/mg; specific activity 15.00 U/mg
Rubrivivax gelatinosus
Storage Stability
Storage Stability
Organism
-20C, partly purified enzyme retains full activity for at least 1 year
Rubrivivax gelatinosus
-20C, purified enzyme loses 20% of activity within 1 year during storage
Rubrivivax gelatinosus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
[citrate (pro-3S)-lyase](acetyl form) + H2O
-
643851
Rubrivivax gelatinosus
[citrate (pro-3S)-lyase](thiol form) + acetate
-
643851
Rubrivivax gelatinosus
?
[citrate (pro-3S)-lyase](acetyl form) + H2O
regulates the activity of citrate layse by covalent modification
643851
Rubrivivax gelatinosus
[citrate (pro-3S)-lyase](thiol form) + acetate
-
643851
Rubrivivax gelatinosus
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 7300, SDS-PAGE
Rubrivivax gelatinosus
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
50
-
half-life 20 min, effectively protected against heat inactivation by 100 mM L-glutamate, half-life 30 min
Rubrivivax gelatinosus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.12
-
L-glutamate
pH 7.5, 30C
Rubrivivax gelatinosus
General Stability (protein specific)
General Stability
Organism
loses activity upon repeated freezing and thawing
Rubrivivax gelatinosus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
alpha-glutamyl-glutamate
10 mM, 70% inhibition
Rubrivivax gelatinosus
D-glutamate
10 mM, 54% inhibition
Rubrivivax gelatinosus
gamma-glutamyl-glutamate
10 mM, 70% inhibition
Rubrivivax gelatinosus
L-aspartate
10 mM, 60% inhibition
Rubrivivax gelatinosus
L-glutamate
linear mixed type of inhibition
Rubrivivax gelatinosus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.12
-
L-glutamate
pH 7.5, 30C
Rubrivivax gelatinosus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
7300
-
2 * 7300, SDS-PAGE
Rubrivivax gelatinosus
14300
-
gel filtration
Rubrivivax gelatinosus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
[citrate (pro-3S)-lyase](acetyl form) + H2O
Rubrivivax gelatinosus
regulates the activity of citrate layse by covalent modification
[citrate (pro-3S)-lyase](thiol form) + acetate
-
Rubrivivax gelatinosus
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rubrivivax gelatinosus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
specific activity 1000 U/mg; specific activity 15.00 U/mg
Rubrivivax gelatinosus
Storage Stability (protein specific)
Storage Stability
Organism
-20C, partly purified enzyme retains full activity for at least 1 year
Rubrivivax gelatinosus
-20C, purified enzyme loses 20% of activity within 1 year during storage
Rubrivivax gelatinosus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
[citrate (pro-3S)-lyase](acetyl form) + H2O
-
643851
Rubrivivax gelatinosus
[citrate (pro-3S)-lyase](thiol form) + acetate
-
643851
Rubrivivax gelatinosus
?
[citrate (pro-3S)-lyase](acetyl form) + H2O
regulates the activity of citrate layse by covalent modification
643851
Rubrivivax gelatinosus
[citrate (pro-3S)-lyase](thiol form) + acetate
-
643851
Rubrivivax gelatinosus
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 7300, SDS-PAGE
Rubrivivax gelatinosus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
50
-
half-life 20 min, effectively protected against heat inactivation by 100 mM L-glutamate, half-life 30 min
Rubrivivax gelatinosus
Other publictions for EC 3.1.2.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
643852
Giffhorn
Substrate specificity of citra ...
no activity in Clostridium sphenoides, Rhodopseudomonas palustris, Rubrivivax gelatinosus
J. Bacteriol.
147
463-470
1981
-
-
-
-
-
-
2
-
-
-
-
2
-
9
-
-
2
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
643851
Giffhorn
Citrate lyase deacetylase of R ...
no activity in Enterobacter aerogenes, no activity in Escherichia coli, no activity in Streptococcus diacetilactis, Rubrivivax gelatinosus
Eur. J. Biochem.
111
461-471
1980
-
-
-
-
-
1
5
-
-
-
2
1
-
6
-
-
1
-
-
-
1
2
2
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
5
1
-
-
-
2
1
-
-
-
1
-
-
1
2
2
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
643850
Giffhorn
Inactivation of citrate lyase ...
no activity in Enterobacter aerogenes, no activity in Streptococcus diacetilactis, Rubrivivax gelatinosus
J. Bacteriol.
124
1052-1061
1975
1
-
-
-
-
-
5
1
-
-
-
1
-
8
-
-
1
-
-
-
-
1
2
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
5
-
1
-
-
-
1
-
-
-
1
-
-
-
1
2
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-