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Literature summary for 3.1.2.14 extracted from

  • Jing, F.; Zhao, L.; Yandeau-Nelson, M.D.; Nikolau, B.J.
    Two distinct domains contribute to the substrate acyl chain length selectivity of plant acyl-ACP thioesterase (2018), Nat. Commun., 9, 860 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.2.14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain K27 Cuphea viscosissima

Protein Variants

Protein Variants Comment Organism
A276P/D282N/L289F the mutation significantly increases the fatty acid titer of the mutant strain Cuphea viscosissima
I260L the mutation significantly increases (by up to 3.5fold) the fatty acid titer of the mutant strain Cuphea viscosissima
L257F the mutation significantly increases (by up to 3.5fold) the fatty acid titer of the mutant strain Cuphea viscosissima
L289F the mutation significantly increases (by up to 2fold) the fatty acid titer of the mutant strain Cuphea viscosissima
V194F the mutant of isoform FatB2 displays distinct substrate specificity toward 4- to 8-carbon acyl-[acyl-carrier protein] Cuphea viscosissima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an oleoyl-[acyl-carrier protein] + H2O Cuphea viscosissima
-
 an [acyl-carrier protein] + oleate
-
 ?

Organism

Organism UniProt Comment Textmining
Cuphea viscosissima G3ESU9
-
-
Cuphea viscosissima G3ESV0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
an oleoyl-[acyl-carrier protein] + H2O
-
 Cuphea viscosissima an [acyl-carrier protein] + oleate
-
 ?
decanoyl-[acyl-carrier protein] + H2O substrate for isoform FatB1 Cuphea viscosissima [acyl-carrier protein] + decanoate
-
 ?
myristoyl-[acyl-carrier protein] + H2O substrate for isoform FatB2 Cuphea viscosissima [acyl-carrier protein] + myristate
-
 ?
nonanoyl-[acyl-carrier protein] + H2O substrate for isoform FatB1 Cuphea viscosissima [acyl-carrier protein] + nonanoate
-
 ?
octanoyl-[acyl-carrier protein] + H2O substrate for isoform FatB1 Cuphea viscosissima [acyl-carrier protein] + octanoate
-
 ?
palmitoyl-[acyl-carrier protein] + H2O substrate for isoform FatB2 Cuphea viscosissima [acyl-carrier protein] + palmitate
-
 ?

Synonyms

Synonyms Comment Organism
acyl-ACP-thioesterase
-
 Cuphea viscosissima
FATB1 isoform Cuphea viscosissima
FatB2 isoform Cuphea viscosissima