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Literature summary for 3.1.2.14 extracted from
- Identification of novel acyl-ACP thioesterase gene ClFATB1 from Cinnamomum longepaniculatum (2013), Biochemistry, 78, 1298-1303.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinantly expressed in Escherichia coli | Cinnamomum longipaniculatum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | - |
SDS-PAGE, recombinant protein | Cinnamomum longipaniculatum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cinnamomum longipaniculatum | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Cinnamomum longipaniculatum | - |
| root | - |
Cinnamomum longipaniculatum | - |
| seed | - |
Cinnamomum longipaniculatum | - |
| stem | - |
Cinnamomum longipaniculatum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | recombinant ClFATB1 in Escherichia coli has higher specific activities against saturated 16:0- and 18:0-ACPs than on unsaturated 18:1-ACP | Cinnamomum longipaniculatum | ? | - |
? |  |
| oleoyl-[acyl-carrier protein] + H2O | - |
Cinnamomum longipaniculatum | oleic acid + acyl-carrier protein | - |
? | |
| palmitoleoyl-[acyl-carrier protein] + H2O | - |
Cinnamomum longipaniculatum | palmitoleic acid + acyl-carrier protein | - |
? | |
| stearoyl-ACP + H2O | - |
Cinnamomum longipaniculatum | stearate + acyl-carrier protein | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ClFATB1 | - |
Cinnamomum longipaniculatum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Cinnamomum longipaniculatum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Cinnamomum longipaniculatum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | overexpression of ClFATB1 in transgenic tobaccos upregulates thioesterase activities of crude proteins against 16:0-ACP and 18:0-ACP by 20.3 and 5.7%, respectively, and results in an increase in the contents of palmitic and stearic acids by 15.4 and 10.5%, respectively. Ectopic expression of this gene decreases the substrate specificities of crude proteins to unsaturated 18:1-ACP by 12.7% in transgenic tobacco and lowers the contents of oleic, linoleic, and linolenic acids in transgenic leaves | Cinnamomum longipaniculatum |
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Release 2023.1 (January 2023)
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