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Literature summary for 3.1.2.14 extracted from
- Cloning, characterization and structural model of a FatA-type thioesterase from sunflower seeds (Helianthus annuus L.) (2005), Planta, 221, 868-880.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene fatA1, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression as soluble His-tagged enzyme in Escherichia coli strain BL21 and DH5alpha, lipid analysis in recombinant Escherichia coli cells | Helianthus annuus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K216A | site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview | Helianthus annuus |
| K216E | site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview | Helianthus annuus |
| R143A/K144A | site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview | Helianthus annuus |
| R143E/K144E | site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview | Helianthus annuus |
| R212A/R213A | site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview | Helianthus annuus |
| R212E/R213E | site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview | Helianthus annuus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0006 | - |
(9Z)-hexadec-9-enoyl-[acyl-carrier protein] | pH 8.0, 22°C, recombinant His-tagged enzyme | Helianthus annuus |  |
| 0.0009 | - |
stearoyl-[acyl-carrier protein] | pH 8.0, 22°C, recombinant His-tagged enzyme | Helianthus annuus | |
| 0.0013 | - |
palmitoyl-[acyl-carrier protein] | pH 8.0, 22°C, recombinant His-tagged enzyme | Helianthus annuus | |
| 0.0021 | - |
oleoyl-[acyl-carrier protein] | pH 8.0, 22°C, recombinant His-tagged enzyme | Helianthus annuus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36900 | - |
x * 41200, about, amino acid sequence calculation, x * 36900, recombinant His-tagged enzyme without signal peptide, SDS-PAGE | Helianthus annuus |
| 41200 | - |
x * 41200, about, amino acid sequence calculation, x * 36900, recombinant His-tagged enzyme without signal peptide, SDS-PAGE | Helianthus annuus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Helianthus annuus | the enzyme is responsible for the biosynthesis of storage and membrane lipids in seeds | ? | - |
? | |
| additional information | Helianthus annuus RHA-274 | the enzyme is responsible for the biosynthesis of storage and membrane lipids in seeds | ? | - |
? | |
| oleoyl-[acyl-carrier protein] + H2O | Helianthus annuus | preferred substrate | [acyl-carrier protein] + oleate | - |
? | |
| oleoyl-[acyl-carrier protein] + H2O | Helianthus annuus RHA-274 | preferred substrate | [acyl-carrier protein] + oleate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helianthus annuus | - |
gene fatA1 | - |
| Helianthus annuus RHA-274 | - |
gene fatA1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli by metal affinity chromatography | Helianthus annuus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Helianthus annuus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Helianthus annuus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is responsible for the biosynthesis of storage and membrane lipids in seeds | Helianthus annuus | ? | - |
? | |
| additional information | substrate specificity of recombinant enzyme, the enzyme shows preference for monounsaturated acyl-ACPs | Helianthus annuus | ? | - |
? | |
| additional information | the enzyme is responsible for the biosynthesis of storage and membrane lipids in seeds | Helianthus annuus RHA-274 | ? | - |
? | |
| additional information | substrate specificity of recombinant enzyme, the enzyme shows preference for monounsaturated acyl-ACPs | Helianthus annuus RHA-274 | ? | - |
? | |
| oleoyl-[acyl-carrier protein] + H2O | preferred substrate | Helianthus annuus | [acyl-carrier protein] + oleate | - |
? | |
| oleoyl-[acyl-carrier protein] + H2O | preferred substrate of wild-type and recombinant enzyme | Helianthus annuus | [acyl-carrier protein] + oleate | - |
? | |
| oleoyl-[acyl-carrier protein] + H2O | preferred substrate | Helianthus annuus RHA-274 | [acyl-carrier protein] + oleate | - |
? | |
| oleoyl-[acyl-carrier protein] + H2O | preferred substrate of wild-type and recombinant enzyme | Helianthus annuus RHA-274 | [acyl-carrier protein] + oleate | - |
? | |
| palmitoleoyl-[acyl-carrier protein] + H2O | - |
Helianthus annuus | [acyl-carrier protein] + palmitoleate | - |
? | |
| palmitoleoyl-[acyl-carrier protein] + H2O | - |
Helianthus annuus RHA-274 | [acyl-carrier protein] + palmitoleate | - |
? | |
| palmitoyl-[acyl-carrier protein] + H2O | - |
Helianthus annuus | [acyl-carrier protein] + palmitate | - |
? | |
| stearoyl-[acyl-carrier protein] + H2O | - |
Helianthus annuus | [acyl-carrier protein] + stearate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 41200, about, amino acid sequence calculation, x * 36900, recombinant His-tagged enzyme without signal peptide, SDS-PAGE | Helianthus annuus |
| More | secondary structure and predicted three-dimensional structure, modeling and analysis | Helianthus annuus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acyl-ACP thioesterase | - |
Helianthus annuus |
| acyl-acyl carrier protein thioesterase | - |
Helianthus annuus |
| FatA-type thioesterase | - |
Helianthus annuus |
| FatA1 thioesterase | - |
Helianthus annuus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Helianthus annuus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
stearoyl-[acyl-carrier protein] | pH 8.0, 22°C, recombinant His-tagged enzyme | Helianthus annuus | |
| 0.5 | - |
palmitoyl-[acyl-carrier protein] | pH 8.0, 22°C, recombinant His-tagged enzyme | Helianthus annuus | |
| 0.9 | - |
(9Z)-hexadec-9-enoyl-[acyl-carrier protein] | pH 8.0, 22°C, recombinant His-tagged enzyme | Helianthus annuus | |
| 11.4 | - |
oleoyl-[acyl-carrier protein] | pH 8.0, 22°C, recombinant His-tagged enzyme | Helianthus annuus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 9.7 | - |
Helianthus annuus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Helianthus annuus | amino acid sequence calculation | - |
8.1 |
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