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Literature summary for 3.1.2.14 extracted from
- Acyl-acyl carrier protein thioesterase activity from sunflower (Helianthus annuus L.) seeds (2000), Planta, 211, 673-678.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Helianthus annuus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| oleoyl-[acyl-carrier protein] | competitive to other acyl-[acyl-carrier protein] substrates | Helianthus annuus |  |
| palmitoyl-[acyl-carrier protein] | competitive to other acyl-[acyl-carrier protein] substrates | Helianthus annuus | |
| stearoyl-[acyl-carrier protein] | competitive to other acyl-[acyl-carrier protein] substrates | Helianthus annuus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00028 | - |
palmitoyl-[acyl-carrier protein] | pH 8.5, 25°C, 15 days after flowering | Helianthus annuus | |
| 0.00034 | - |
stearoyl-[acyl-carrier protein] | pH 8.5, 25°C, 15 days after flowering | Helianthus annuus | |
| 0.00053 | - |
palmitoyl-[acyl-carrier protein] | pH 8.5, 25°C, 30 days after flowering | Helianthus annuus | |
| 0.00074 | - |
oleoyl-[acyl-carrier protein] | pH 8.5, 25°C, 30 days after flowering | Helianthus annuus | |
| 0.0008 | - |
oleoyl-[acyl-carrier protein] | pH 8.5, 25°C, 15 days after flowering | Helianthus annuus | |
| 0.00092 | - |
stearoyl-[acyl-carrier protein] | pH 8.5, 25°C, 30 days after flowering | Helianthus annuus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oleoyl-[acyl-carrier protein] + H2O | Helianthus annuus | - |
oleic acid + acyl-carrier protein | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helianthus annuus | - |
line RHA-274 with normal fatty acid content and high-palmitic seed oil line CAS-5, 2 isozymes: FatA type and FatB type | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | highest enzyme activity during seed development 15 days after flowering | Helianthus annuus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oleoyl-[acyl-carrier protein] + H2O | - |
Helianthus annuus | oleic acid + acyl-carrier protein | - |
? | |
| oleoyl-[acyl-carrier protein] + H2O | preferred substrate of FatA type isozyme, low activity with FatB type isozyme | Helianthus annuus | oleic acid + acyl-carrier protein | - |
? | |
| palmitoyl-[acyl-carrier protein] + H2O | preferred substrate of FatB type isozyme | Helianthus annuus | palmitic acid + acyl-carrier protein | - |
? | |
| stearoyl-[acyl-carrier protein] + H2O | preferred substrate of FatB type isozyme, low activity with FatA type isozyme | Helianthus annuus | stearic acid + acyl-carrier protein | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 12:0-ACP thioesterase | - |
Helianthus annuus |
| 12:0-acyl-carrier protein thioesterase | - |
Helianthus annuus |
| 14:0-ACP thioesterase | - |
Helianthus annuus |
| 14:0-acyl-carrier protein thioesterase | - |
Helianthus annuus |
| 16:0-ACP thioesterase | - |
Helianthus annuus |
| 16:0-acyl-carrier protein thioesterase | - |
Helianthus annuus |
| 18:0-ACP thioesterase | - |
Helianthus annuus |
| 18:0-acyl-carrier protein thioesterase | - |
Helianthus annuus |
| acyl-ACP-hydrolase | - |
Helianthus annuus |
| acyl-acyl carrier protein hydrolase | - |
Helianthus annuus |
| acyl-acyl carrier protein thioesterase | - |
Helianthus annuus |
| acyl-[acyl-carrier-protein] hydrolase | - |
Helianthus annuus |
| AH | - |
Helianthus annuus |
| BTE | - |
Helianthus annuus |
| C12:0 ACP TE | - |
Helianthus annuus |
| C18:1-ACP thioesterase | - |
Helianthus annuus |
| Deubiquitinating enzyme 14 | - |
Helianthus annuus |
| hydrolase, oleoyl-[acyl carrier protein] | - |
Helianthus annuus |
| oleoyl-ACP thioesterase | - |
Helianthus annuus |
| oleoyl-acyl carrier protein thioesterase | - |
Helianthus annuus |
| OTE | - |
Helianthus annuus |
| PATE | - |
Helianthus annuus |
| STE | - |
Helianthus annuus |
| Ubiquitin thiolesterase 14 | - |
Helianthus annuus |
| Ubiquitin-specific processing protease 14 | - |
Helianthus annuus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Helianthus annuus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Helianthus annuus |
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