BRENDA - Enzyme Database
show all sequences of 3.1.2.14

Identification of a heat-stable palmitoyl/oleoyl specific acyl-acyl carrier protein thioesterase in developing seeds of Madhuca butyracea

Bhattacharjee, A.; Ghosh, S.K.; Ghosh, D.; Ghosh, S.; Maiti, M.K.; Sen, S.K.; Plant Sci. 163, 791-800 (2002)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
glycerol, 25%, is required for stability during purification for the enzyme form PO-FAT
Diploknema butyracea
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0034
-
stearoyl-[acyl-carrier protein]
enzyme form PO-FAT, pH 9.0, 37C
Diploknema butyracea
0.0036
-
palmitoyl-[acyl-carrier protein]
enzyme form PO-FAT, pH 9.0, 37C
Diploknema butyracea
0.008
-
oleoyl-[acyl-carrier protein]
enzyme form PO-FAT, pH 9.0, 37C
Diploknema butyracea
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
gel filtration
Diploknema butyracea
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
oleoyl-[acyl-carrier protein] + H2O
Diploknema butyracea
-
oleic acid + acyl-carrier protein
-
Diploknema butyracea
?
palmitoyl-[acyl-carrier protein] + H2O
Diploknema butyracea
-
palmitic acid + acyl-carrier protein
-
Diploknema butyracea
?
Organism
Organism
UniProt
Commentary
Textmining
Diploknema butyracea
-
i.e. Brassica butyracea, 2 enzyme forms: a palmitoyl-/oleoyl-[acyl carrier protein] specific and a oleoyl-[acyl carrier protein] specific one, i.e. PO-FAT and LC-FAT, respectively
-
Purification (Commentary)
Purification (Commentary)
Organism
enzyme form PO-FAT, 499fold
Diploknema butyracea
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower bud
-
Diploknema butyracea
-
leaf
young
Diploknema butyracea
-
seed
developing, period from 45 to 93 days after flowering
Diploknema butyracea
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Diploknema butyracea
0.69
-
seed 65 days after flowering, enzyme form PO-FAT
Diploknema butyracea
192
-
purified enzyme form PO-FAT
Diploknema butyracea
Storage Stability
Storage Stability
Organism
-20C, enzyme form PO-FAT, ammonium sulfate pellet, stable
Diploknema butyracea
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
oleoyl-[acyl-carrier protein] + H2O
-
653557
Diploknema butyracea
oleic acid + acyl-carrier protein
-
653557
Diploknema butyracea
?
oleoyl-[acyl-carrier protein] + H2O
preferred substrate of the oleoyl-[acyl carrier protein] specific enzyme form LC-FAT
653557
Diploknema butyracea
oleic acid + acyl-carrier protein
-
653557
Diploknema butyracea
?
palmitoyl-[acyl-carrier protein] + H2O
-
653557
Diploknema butyracea
palmitic acid + acyl-carrier protein
-
653557
Diploknema butyracea
?
palmitoyl-[acyl-carrier protein] + H2O
preferred substrate of the palmitoyl-/oleoyl-[acyl carrier protein] specific enzyme form PO-FAT
653557
Diploknema butyracea
palmitic acid + acyl-carrier protein
-
653557
Diploknema butyracea
?
stearoyl-[acyl-carrier protein] + H2O
-
653557
Diploknema butyracea
stearic acid + acyl-carrier protein
-
653557
Diploknema butyracea
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 42500-44000, SDS-PAGE
Diploknema butyracea
Synonyms
Synonyms
Commentary
Organism
12:0-ACP thioesterase
-
Diploknema butyracea
12:0-acyl-carrier protein thioesterase
-
Diploknema butyracea
14:0-ACP thioesterase
-
Diploknema butyracea
14:0-acyl-carrier protein thioesterase
-
Diploknema butyracea
16:0-ACP thioesterase
-
Diploknema butyracea
16:0-acyl-carrier protein thioesterase
-
Diploknema butyracea
18:0-ACP thioesterase
-
Diploknema butyracea
18:0-acyl-carrier protein thioesterase
-
Diploknema butyracea
acyl-ACP-hydrolase
-
Diploknema butyracea
acyl-acyl carrier protein hydrolase
-
Diploknema butyracea
acyl-acyl carrier protein thioesterase
-
Diploknema butyracea
acyl-[acyl-carrier-protein] hydrolase
-
Diploknema butyracea
AH
-
Diploknema butyracea
BTE
-
Diploknema butyracea
C12:0 ACP TE
-
Diploknema butyracea
C18:1-ACP thioesterase
-
Diploknema butyracea
Deubiquitinating enzyme 14
-
Diploknema butyracea
FAT
-
Diploknema butyracea
fatty acyl-ACP thioesterase
-
Diploknema butyracea
hydrolase, oleoyl-[acyl carrier protein]
-
Diploknema butyracea
LC-FAT
oleoyl-[acyl carrier protein] specific
Diploknema butyracea
oleoyl-ACP thioesterase
-
Diploknema butyracea
oleoyl-acyl carrier protein thioesterase
-
Diploknema butyracea
OTE
-
Diploknema butyracea
PATE
-
Diploknema butyracea
PO-FAT
palmitoyl-/oleoyl-[acyl carrier protein] specific
Diploknema butyracea
STE
-
Diploknema butyracea
Ubiquitin thiolesterase 14
-
Diploknema butyracea
Ubiquitin-specific processing protease 14
-
Diploknema butyracea
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Diploknema butyracea
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
60
-
15 min, enzyme form LC-FAT is mostly inactivated, while enzyme form PO-FAT is activated by 15-20%
Diploknema butyracea
70
-
15 min, enzyme form PO-FAT is heat-stable, after 45 min the enzyme retains 30% of the original activity
Diploknema butyracea
80
-
15 min, 30% remaining activity
Diploknema butyracea
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
enzyme form PO-FAT
Diploknema butyracea
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Diploknema butyracea
enzyme form LC-FAT, chromatofocusing
-
8.2
Diploknema butyracea
enzyme form PO-FAT, chromatofocusing
-
9.7
General Stability (protein specific)
General Stability
Organism
glycerol, 25%, is required for stability during purification for the enzyme form PO-FAT
Diploknema butyracea
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0034
-
stearoyl-[acyl-carrier protein]
enzyme form PO-FAT, pH 9.0, 37C
Diploknema butyracea
0.0036
-
palmitoyl-[acyl-carrier protein]
enzyme form PO-FAT, pH 9.0, 37C
Diploknema butyracea
0.008
-
oleoyl-[acyl-carrier protein]
enzyme form PO-FAT, pH 9.0, 37C
Diploknema butyracea
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
gel filtration
Diploknema butyracea
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
oleoyl-[acyl-carrier protein] + H2O
Diploknema butyracea
-
oleic acid + acyl-carrier protein
-
Diploknema butyracea
?
palmitoyl-[acyl-carrier protein] + H2O
Diploknema butyracea
-
palmitic acid + acyl-carrier protein
-
Diploknema butyracea
?
Purification (Commentary) (protein specific)
Commentary
Organism
enzyme form PO-FAT, 499fold
Diploknema butyracea
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower bud
-
Diploknema butyracea
-
leaf
young
Diploknema butyracea
-
seed
developing, period from 45 to 93 days after flowering
Diploknema butyracea
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Diploknema butyracea
0.69
-
seed 65 days after flowering, enzyme form PO-FAT
Diploknema butyracea
192
-
purified enzyme form PO-FAT
Diploknema butyracea
Storage Stability (protein specific)
Storage Stability
Organism
-20C, enzyme form PO-FAT, ammonium sulfate pellet, stable
Diploknema butyracea
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
oleoyl-[acyl-carrier protein] + H2O
-
653557
Diploknema butyracea
oleic acid + acyl-carrier protein
-
653557
Diploknema butyracea
?
oleoyl-[acyl-carrier protein] + H2O
preferred substrate of the oleoyl-[acyl carrier protein] specific enzyme form LC-FAT
653557
Diploknema butyracea
oleic acid + acyl-carrier protein
-
653557
Diploknema butyracea
?
palmitoyl-[acyl-carrier protein] + H2O
-
653557
Diploknema butyracea
palmitic acid + acyl-carrier protein
-
653557
Diploknema butyracea
?
palmitoyl-[acyl-carrier protein] + H2O
preferred substrate of the palmitoyl-/oleoyl-[acyl carrier protein] specific enzyme form PO-FAT
653557
Diploknema butyracea
palmitic acid + acyl-carrier protein
-
653557
Diploknema butyracea
?
stearoyl-[acyl-carrier protein] + H2O
-
653557
Diploknema butyracea
stearic acid + acyl-carrier protein
-
653557
Diploknema butyracea
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 42500-44000, SDS-PAGE
Diploknema butyracea
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Diploknema butyracea
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
60
-
15 min, enzyme form LC-FAT is mostly inactivated, while enzyme form PO-FAT is activated by 15-20%
Diploknema butyracea
70
-
15 min, enzyme form PO-FAT is heat-stable, after 45 min the enzyme retains 30% of the original activity
Diploknema butyracea
80
-
15 min, 30% remaining activity
Diploknema butyracea
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
enzyme form PO-FAT
Diploknema butyracea
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Diploknema butyracea
enzyme form LC-FAT, chromatofocusing
-
8.2
Diploknema butyracea
enzyme form PO-FAT, chromatofocusing
-
9.7
Other publictions for EC 3.1.2.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749480
Hernandez Lozada
Highly active C8-acyl-ACP thi ...
Cuphea palustris
ACS Synth. Biol.
7
2205-2215
2018
-
-
1
-
8
-
-
1
-
-
-
1
-
4
-
-
1
-
-
-
-
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1
-
2
-
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1
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1
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8
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1
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1
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1
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1
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-
-
1
-
-
-
-
-
-
-
-
1
1
749676
Ziesack
Chimeric fatty acyl-acyl carr ...
Cuphea palustris
Appl. Environ. Microbiol.
84
e02868-17
2018
-
-
1
-
6
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
1
2
-
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1
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6
-
-
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1
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
749700
Feng
Tuning of acyl-ACP thioestera ...
Cuphea hookeriana
Appl. Microbiol. Biotechnol.
102
3173-3182
2018
-
-
1
-
2
-
-
3
-
-
-
1
-
2
-
-
1
-
-
-
-
-
3
-
2
-
-
-
3
-
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-
-
-
-
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-
1
-
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2
-
-
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3
-
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1
-
-
-
1
-
-
-
-
3
-
-
-
-
3
-
-
-
-
-
-
-
-
3
3
749859
Jing
Identification of active site ...
Cocos nucifera, Cuphea viscosissima
Biochem. J.
475
3861-3873
2018
-
-
2
-
22
-
-
-
-
-
-
3
-
8
-
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2
-
-
-
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-
3
-
12
-
-
-
-
-
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3
-
-
22
-
-
-
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3
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3
-
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3
-
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-
-
-
-
-
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750653
Lin
Cellular and molecular respon ...
Cinnamomum camphora
Front. Microbiol.
9
619
2018
-
-
1
-
-
-
-
-
-
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3
-
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2
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3
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1
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2
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750918
Bilyeu
-
The interaction of the soybea ...
Glycine max
J. Am. Oil Chem. Soc.
95
39-49
2018
-
-
-
-
-
-
-
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1
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1
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2
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751676
Jing
Two distinct domains contribu ...
Cuphea viscosissima
Nat. Commun.
9
860
2018
-
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1
-
5
-
-
-
-
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2
-
4
-
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7
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4
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2
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5
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2
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-
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7
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751804
Campe
A new herbicidal site of acti ...
Lemna
Pestic. Biochem. Physiol.
148
116-125
2018
-
-
1
1
-
-
1
-
1
-
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1
-
4
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1
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4
-
3
-
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1
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1
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1
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1
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1
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4
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751864
Brands
The Lotus japonicus acyl-acyl ...
Lotus japonicus
Plant J.
95
219-232
2018
-
-
1
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-
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1
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1
-
6
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2
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5
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3
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1
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1
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2
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5
-
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-
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1
1
-
-
-
751885
Kalinger
Elucidating the substrate spe ...
Arabidopsis thaliana, Zea mays
Plant Physiol. Biochem.
127
104-118
2018
-
-
2
-
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2
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2
-
9
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7
-
7
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2
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7
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749461
Feng
Structural insight into acyl- ...
Umbellularia californica
ACS Chem. Biol.
12
2830-2836
2017
-
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1
1
8
-
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1
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1
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2
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1
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5
1
3
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1
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1
8
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1
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1
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1
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5
1
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751156
Tan
Expression of the heterologou ...
Dunaliella tertiolecta
J. Biotechnol.
247
60-67
2017
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1
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6
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751897
Aznar-Moreno
Acyl carrier proteins from su ...
Helianthus annuus
Planta
244
479-490
2016
-
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1
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12
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4
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1
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1
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3
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3
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12
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12
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731878
Dong
Cloning, characterization, and ...
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Rodriguez-Rodriguez
Acyl-ACP thioesterases from Ca ...
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Phytochemistry
107
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Identification of novel acyl-A ...
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Bhattacharjee
Deposition of stearate-oleate ...
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Dani
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Acyl-ACP thioesterases from ca ...
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Ghosh
Characterization and cloning o ...
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Cloning and functional express ...
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Mayer
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Serrano-Vega
Cloning, characterization and ...
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Asemota
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Acyl-acyl carrier protein (ACP ...
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Huynh
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Expression of a Gossypium hirs ...
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Palmitoyl-acyl carrier protein ...
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