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Literature summary for 3.1.2.14 extracted from

  • Othman, A.; Lazarus, C.; Fraser, T.; Stobart, K.
    Cloning of a palmitoyl-acyl carrier protein thioesterase from oil palm (2000), Biochem. Soc. Trans., 28, 619-622.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
single gene, partial DNA sequence determination and DNA sequence analysis, expression in Escherichia coli BL21 (DE3) as glutathione S-transferase fusion protein Elaeis sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information the gene contains a putative transit peptide sequence Elaeis sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
x * 66000, recombinant glutathione S-transferase fusion protein, SDS-PAGE Elaeis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oleoyl-[acyl-carrier protein] + H2O Elaeis sp.
-
oleic acid + acyl-carrier protein
-
?
palmitoyl-[acyl-carrier protein] + H2O Elaeis sp. enzyme plays an important role in the control of seed-oil composition, determination of fatty acid chain growth palmitic acid + acyl-carrier protein
-
?

Organism

Organism UniProt Comment Textmining
Elaeis sp.
-
oil palm
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Elaeis sp.
-
leaf high level expression Elaeis sp.
-
mesocarp 15-18 weeks old tissue, high level expression, 8-12 weeks old tissue, low level expression Elaeis sp.
-
additional information no expression in kernel tissues Elaeis sp.
-
seedling germinating, low level expression Elaeis sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lauroyl-[acyl-carrier protein] + H2O low activity Elaeis sp. lauric acid + acyl-carrier protein
-
?
additional information the recombinant enzyme hydrolyzes medium- and long-chain acyl-CoAs with a 10fold lower activity compared to acyl-[acyl-carrier-protein]s Elaeis sp. ?
-
?
myristoyl-[acyl-carrier protein] + H2O
-
Elaeis sp. myristic acid + acyl-carrier protein
-
?
oleoyl-[acyl-carrier protein] + H2O
-
Elaeis sp. oleic acid + acyl-carrier protein
-
?
palmitoyl-[acyl-carrier protein] + H2O best substrate Elaeis sp. palmitic acid + acyl-carrier protein
-
?
palmitoyl-[acyl-carrier protein] + H2O enzyme plays an important role in the control of seed-oil composition, determination of fatty acid chain growth Elaeis sp. palmitic acid + acyl-carrier protein
-
?
stearoyl-[acyl-carrier protein] + H2O
-
Elaeis sp. stearic acid + acyl-carrier protein
-
?

Subunits

Subunits Comment Organism
? x * 66000, recombinant glutathione S-transferase fusion protein, SDS-PAGE Elaeis sp.

Synonyms

Synonyms Comment Organism
12:0-ACP thioesterase
-
Elaeis sp.
12:0-acyl-carrier protein thioesterase
-
Elaeis sp.
14:0-ACP thioesterase
-
Elaeis sp.
14:0-acyl-carrier protein thioesterase
-
Elaeis sp.
16:0-ACP thioesterase
-
Elaeis sp.
16:0-acyl-carrier protein thioesterase
-
Elaeis sp.
18:0-ACP thioesterase
-
Elaeis sp.
18:0-acyl-carrier protein thioesterase
-
Elaeis sp.
acyl-ACP-hydrolase
-
Elaeis sp.
acyl-acyl carrier protein hydrolase
-
Elaeis sp.
acyl-[acyl-carrier-protein] hydrolase
-
Elaeis sp.
AH
-
Elaeis sp.
BTE
-
Elaeis sp.
C12:0 ACP TE
-
Elaeis sp.
C18:1-ACP thioesterase
-
Elaeis sp.
Deubiquitinating enzyme 14
-
Elaeis sp.
hydrolase, oleoyl-[acyl carrier protein]
-
Elaeis sp.
oleoyl-ACP thioesterase
-
Elaeis sp.
oleoyl-acyl carrier protein thioesterase
-
Elaeis sp.
OTE
-
Elaeis sp.
palmitoyl-acyl carrier protein thioesterase
-
Elaeis sp.
PATE
-
Elaeis sp.
STE
-
Elaeis sp.
Ubiquitin thiolesterase 14
-
Elaeis sp.
Ubiquitin-specific processing protease 14
-
Elaeis sp.