Literature summary for 3.1.2.12 extracted from

van Straaten, K.E.; Gonzalez, C.F.; Valladares, R.B.; Xu, X.; Savchenko, A.V.; Sanders, D.A.
The structure of a putative S-formylglutathione hydrolase from Agrobacterium tumefaciens (2009), Protein Sci., 18, 2196-2202.

Search for more literature for 3.1.2.12

Cloned(Commentary)

Cloned (Comment)	Organism
-	Agrobacterium tumefaciens

Crystallization (Commentary)

Crystallization (Comment)	Organism
by the sitting drop-vapor diffusion technique, at 2 A resolution. Crystals belong to space group P1 with unit cell dimensions a = 66.43 A, b = 68.95 A, c = 95.65 A, alpha = 92.41, beta = 99.79, gamma = 98.43. Contacts between the two monomers in the dimer are formed both by hydrogen bonds and salt bridges	Agrobacterium tumefaciens

Protein Variants

Protein Variants	Comment	Organism
C54S	shows a 4fold decrease in alpha-naphthyl butyrate activity. Is insensitive to the inhibitor N-ethylmaleimide	Agrobacterium tumefaciens
S147A	is unable to hydrolyse alpha-naphthyl butyrate	Agrobacterium tumefaciens

Inhibitors

Inhibitors	Comment	Organism	Structure
N-ethylmaleimide	binding of the inhibitor molecule to the conserved cysteine residue Cys54 located in the active site of the enzyme, consequently, blocking the binding of substrate to the active site	Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.02	-	4-nitrophenyl caproate	in Tris-HCl buffer, pH 8.0	Agrobacterium tumefaciens
0.05	-	4-nitrophenyl propionate	in Tris-HCl buffer, pH 8.0	Agrobacterium tumefaciens
0.11	-	4-nitrophenyl butyrate	in Tris-HCl buffer, pH 8.0	Agrobacterium tumefaciens
0.35	-	alpha-naphthyl propionate	in Tris-HCl buffer, pH 8.0	Agrobacterium tumefaciens
0.44	-	naphthalen-1-yl n-butanoate	in Tris-HCl buffer, pH 8.0	Agrobacterium tumefaciens
0.48	-	4-nitrophenyl acetate	in Tris-HCl buffer, pH 8.0	Agrobacterium tumefaciens

Organism

Organism	UniProt	Comment	Textmining
Agrobacterium tumefaciens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl acetate + H2O	-	Agrobacterium tumefaciens	4-nitrophenol + acetate	-	?
4-nitrophenyl butyrate + H2O	highest activity	Agrobacterium tumefaciens	4-nitrophenol + butyrate	-	?
4-nitrophenyl caproate + H2O	-	Agrobacterium tumefaciens	4-nitrophenol + caproate	-	?
4-nitrophenyl propionate + H2O	-	Agrobacterium tumefaciens	4-nitrophenol + propionate	-	?
alpha-naphthyl butyrate + H2O	-	Agrobacterium tumefaciens	alpha-naphthol + butyrate	-	?
alpha-naphthyl propionate + H2O	-	Agrobacterium tumefaciens	alpha-naphthol + propionate	-	?
additional information	is unable to hydrolyse alpha-naphthyl dodecanoate, 4-nitrophenyl caprate, 4-nitrophenyl laurate, and 4-nitrophenyl palmitate. Potential role for Cys54 in regulation of enzyme activity through S-glutathionylation. Hydrolyzes C-O bonds with high affinity toward short to medium chain esters (C3-C4). Has more affinity toward C3 and C4 4-nitrophenyl esters than toward C3 and C4 alpha-naphthyl esters	Agrobacterium tumefaciens	?	-	?

Subunits

Subunits	Comment	Organism
homodimer	crystallography	Agrobacterium tumefaciens

Synonyms

Synonyms	Comment	Organism
Atu1476	-	Agrobacterium tumefaciens
S-formylglutathione hydrolase	-	Agrobacterium tumefaciens
SFGH	-	Agrobacterium tumefaciens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.67	-	4-nitrophenyl caproate	in Tris-HCl buffer, pH 8.0	Agrobacterium tumefaciens
1.73	-	naphthalen-1-yl n-butanoate	in Tris-HCl buffer, pH 8.0	Agrobacterium tumefaciens
1.84	-	4-nitrophenyl propionate	in Tris-HCl buffer, pH 8.0	Agrobacterium tumefaciens
1.9	-	alpha-naphthyl propionate	in Tris-HCl buffer, pH 8.0	Agrobacterium tumefaciens
1.95	-	4-nitrophenyl acetate	in Tris-HCl buffer, pH 8.0	Agrobacterium tumefaciens
4.48	-	4-nitrophenyl butyrate	in Tris-HCl buffer, pH 8.0	Agrobacterium tumefaciens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
3.93	-	naphthalen-1-yl n-butanoate	in Tris-HCl buffer, pH 8.0	Agrobacterium tumefaciens
4.07	-	4-nitrophenyl acetate	in Tris-HCl buffer, pH 8.0	Agrobacterium tumefaciens
5.54	-	alpha-naphthyl propionate	in Tris-HCl buffer, pH 8.0	Agrobacterium tumefaciens
38.5	-	4-nitrophenyl propionate	in Tris-HCl buffer, pH 8.0	Agrobacterium tumefaciens
39.6	-	4-nitrophenyl caproate	in Tris-HCl buffer, pH 8.0	Agrobacterium tumefaciens
40.8	-	4-nitrophenyl butyrate	in Tris-HCl buffer, pH 8.0	Agrobacterium tumefaciens

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Release 2023.1 (January 2023)