Cloned (Comment) | Organism |
---|---|
N-terminus His6-S-tagged protein from pET28a construct overexpressed in Escherichia coli strain BL21(DE3) under the control of the T7 RNA polymerase transcription system | Pseudoalteromonas haloplanktis |
Crystallization (Comment) | Organism |
---|---|
by the hanging drop vapor diffusion technique, to 2.2 A resolution. Crystals belong to space group P212121 with unit cell dimensions of a = 49.49 A, b = 129.75 A, c = 152.67 A, with 4 molecules per asymmetric unit | Pseudoalteromonas haloplanktis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
2 * 30000, SDS-PAGE | Pseudoalteromonas haloplanktis |
60000 | - |
gel filtration | Pseudoalteromonas haloplanktis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas haloplanktis | - |
TAC125 | - |
Pseudoalteromonas haloplanktis TAC 125 | - |
TAC125 | - |
Purification (Comment) | Organism |
---|---|
to homogeneity by a single-step Ni2+ affinity chromatography | Pseudoalteromonas haloplanktis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl thioacetate + H2O | substrate with small acyl moiety | Pseudoalteromonas haloplanktis | 4-nitrophenol + thioacetate | - |
? | |
4-nitrophenyl thioacetate + H2O | substrate with small acyl moiety | Pseudoalteromonas haloplanktis TAC 125 | 4-nitrophenol + thioacetate | - |
? | |
additional information | PhEst contains a catalytic triad formed by residues Ser147, Asp225 and His258. It shows no activity toward substrates with bulky acyl groups such as S-lactoylglutathione. Has a very narrow acyl-binding pocket in a typical alpha/beta-hydrolase fold | Pseudoalteromonas haloplanktis | ? | - |
? | |
additional information | PhEst contains a catalytic triad formed by residues Ser147, Asp225 and His258. It shows no activity toward substrates with bulky acyl groups such as S-lactoylglutathione. Has a very narrow acyl-binding pocket in a typical alpha/beta-hydrolase fold | Pseudoalteromonas haloplanktis TAC 125 | ? | - |
? | |
S-acetylglutathione + H2O | substrate with small acyl moiety, high activity | Pseudoalteromonas haloplanktis | glutathione + acetate | - |
? | |
S-acetylglutathione + H2O | substrate with small acyl moiety, high activity | Pseudoalteromonas haloplanktis TAC 125 | glutathione + acetate | - |
? | |
S-formylglutathione + H2O | substrate with small acyl moiety, lower activity than compared to S-acetylglutathione | Pseudoalteromonas haloplanktis | glutathione + formate | - |
? | |
S-formylglutathione + H2O | substrate with small acyl moiety, lower activity than compared to S-acetylglutathione | Pseudoalteromonas haloplanktis TAC 125 | glutathione + formate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 30000, SDS-PAGE | Pseudoalteromonas haloplanktis |
Synonyms | Comment | Organism |
---|---|---|
FGH | - |
Pseudoalteromonas haloplanktis |
PhEst | - |
Pseudoalteromonas haloplanktis |
S-formylglutathione hydrolase | - |
Pseudoalteromonas haloplanktis |