Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | at time of rewarming 0.5 mM acetyl-CoA restores the activity about 50% | Rattus norvegicus | |
albumin | albumin enhances the reactivation of the monomeric form | Rattus norvegicus | |
ATP | at time of rewarming ATP greatly enhances the restoration of the activity, 2 mM ATP restores the enzyme activity about 70% | Rattus norvegicus | |
diphosphate | 450 mM pyrophosphate restores the activity about 80% at time of rewarming | Rattus norvegicus | |
phosphate | 920 mM phosphate restores the activity about 80% at time of rewarming | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | extramitochondrial | Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
135000 | - |
- |
Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Renatured (Comment) | Organism |
---|---|
from cold inactivation | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Subunits | Comment | Organism |
---|---|---|
monomer | sucrose density gradient centrifugation | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
warming at 37°C after cold exposure is optimal for reactivation | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | purified dimeric enzyme fairly stable in potassium phosphate buffer of pH 7.0-8.0 | Rattus norvegicus |