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Literature summary for 3.1.16.1 extracted from
- Purification of phosphodiesterase II from rat and guinea-pig intestinal mucosa (1976), Biochem. J., 155, 607-613.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | slight inhibition at 0.1 M | Cavia porcellus |  |
| EDTA | slight inhibition at 0.1 M | Rattus norvegicus | |
| high ionic strength | slightly greater inhibition of rat than of guinea-pig enzyme at sodium succinate buffer concentrations greater 0.4 M, also markedly inhibition by 6 M NaCl and 2 M ammonium sulfate | Cavia porcellus | |
| high ionic strength | slightly greater inhibition of rat than of guinea-pig enzyme at sodium succinate buffer concentrations greater 0.4 M, also markedly inhibition by 6 M NaCl and 2 M ammonium sulfate | Rattus norvegicus | |
| Mg2+ | slight inbibition at 0.1 M MgCl2 | Cavia porcellus | |
| Mg2+ | slight inbibition at 0.1 M MgCl2 | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
thymidine 3'-(2,4-dinitrophenyl)phosphate | - |
Cavia porcellus | |
| 0.04 | - |
thymidine 3'-(2,4-dinitrophenyl)phosphate | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lysosome | - |
Cavia porcellus | 5764 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cavia porcellus |
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| intestinal mucosa | homogenates of epithelial cells | Cavia porcellus | - |
| intestinal mucosa | homogenates of epithelial cells | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.123 | - |
- |
Cavia porcellus |
| 3.45 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thymidine 3'-(2,4-dinitrophenyl)phosphate + H2O | - |
Cavia porcellus | thymidine 3'-phosphate + 2,4-dinitrophenol | - |
? | |
| thymidine 3'-(2,4-dinitrophenyl)phosphate + H2O | - |
Rattus norvegicus | thymidine 3'-phosphate + 2,4-dinitrophenol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phosphodiesterase II | - |
Cavia porcellus |
| phosphodiesterase II | - |
Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 61 | - |
lost of 50% of activity after heating of partially purified enzyme for 5 min | Cavia porcellus |
| 64 | - |
lost of 50% of activity after heating of partially purified enzyme for 5 min | Rattus norvegicus |
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