BRENDA - Enzyme Database show
show all sequences of 3.1.16.1

Spleen acid exonuclease

Bernardi, A.; Bernardi, G.; The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ) 4, 329-336 (1971)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
EDTA
with RNA core as substrate
Bos taurus
sulfhydryl reagents
with RNA core as substrate
Bos taurus
Inhibitors
Inhibitors
Commentary
Organism
Structure
acetylation
complete inhibition by acetylation of the 2'-OH of tRNA
Bos taurus
arsenite
weak inhibitor with RNA core as substrate
Bos taurus
Cu2+
-
Bos taurus
Deoxyribonucleoside 3'-phosphates
-
Bos taurus
F-
weak inhibitor with RNA core as substrate
Bos taurus
glycosylation
no activity with glycosylated oligonucleotides obtained from T4 phage DNA
Bos taurus
Hg2+
with RNA core as substrate
Bos taurus
Mg2+
with RNA core as substrate
Bos taurus
Mn2+
with RNA core as substrate
Bos taurus
Zn2+
with RNA core as substrate
Bos taurus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
spleen
-
Bos taurus
-
Storage Stability
Storage Stability
Organism
-15C, in 50% glycerol
Bos taurus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl phosphate
-
134135
Bos taurus
4-nitrophenol + phosphate
-
134135
Bos taurus
?
bis-(4-nitrophenyl)phosphate + H2O
-
134135
Bos taurus
4-nitrophenyl phosphate + 4-nitrophenol
-
134135
Bos taurus
?
DNA + H2O
denatured only, no native or double stranded
134135
Bos taurus
deoxyribonucleoside 3'-phosphates
-
134135
Bos taurus
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.8
-
substrate bis-(p-nitrophenyl) phosphate in 0.25 M succinate buffer
Bos taurus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
EDTA
with RNA core as substrate
Bos taurus
sulfhydryl reagents
with RNA core as substrate
Bos taurus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
acetylation
complete inhibition by acetylation of the 2'-OH of tRNA
Bos taurus
arsenite
weak inhibitor with RNA core as substrate
Bos taurus
Cu2+
-
Bos taurus
Deoxyribonucleoside 3'-phosphates
-
Bos taurus
F-
weak inhibitor with RNA core as substrate
Bos taurus
glycosylation
no activity with glycosylated oligonucleotides obtained from T4 phage DNA
Bos taurus
Hg2+
with RNA core as substrate
Bos taurus
Mg2+
with RNA core as substrate
Bos taurus
Mn2+
with RNA core as substrate
Bos taurus
Zn2+
with RNA core as substrate
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
spleen
-
Bos taurus
-
Storage Stability (protein specific)
Storage Stability
Organism
-15C, in 50% glycerol
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-nitrophenyl phosphate
-
134135
Bos taurus
4-nitrophenol + phosphate
-
134135
Bos taurus
?
bis-(4-nitrophenyl)phosphate + H2O
-
134135
Bos taurus
4-nitrophenyl phosphate + 4-nitrophenol
-
134135
Bos taurus
?
DNA + H2O
denatured only, no native or double stranded
134135
Bos taurus
deoxyribonucleoside 3'-phosphates
-
134135
Bos taurus
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.8
-
substrate bis-(p-nitrophenyl) phosphate in 0.25 M succinate buffer
Bos taurus
Other publictions for EC 3.1.16.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
710529
Gao
Sequence confirmation of chemi ...
Bos taurus
Rapid Commun. Mass Spectrom.
23
3423-3430
2009
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681823
Casta
Expression and biochemical cha ...
Plasmodium falciparum, Plasmodium falciparum FCR-3/C5, Plasmodium yoelii
Mol. Biochem. Parasitol.
157
1-12
2008
-
2
2
-
1
-
3
3
2
5
3
-
-
7
-
-
2
-
-
-
-
-
5
-
-
-
-
3
-
-
-
-
-
-
-
-
2
2
-
-
1
-
-
3
-
3
2
5
3
-
-
-
-
2
-
-
-
-
5
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
690712
Wei
Physical and functional intera ...
Sulfurisphaera tokodaii
Biochem. Biophys. Res. Commun.
367
523-529
2008
-
-
1
-
-
-
1
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
681898
Choudhury
Functional and genetic analysi ...
Saccharomyces cerevisiae
Mol. Cell. Biochem.
300
215-226
2007
-
1
1
-
1
-
1
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
682204
Wojcik
Nucleotide pyrophosphatase/pho ...
Homo sapiens
Oligonucleotides
17
134-145
2007
-
-
-
-
-
-
12
3
-
-
-
-
-
2
-
-
-
-
-
1
-
-
12
-
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
13
12
-
3
-
-
-
-
-
-
-
-
-
1
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
682165
Burkovics
Human Ape2 protein has a 3-5 e ...
Homo sapiens
Nucleic Acids Res.
34
2508-2515
2006
-
1
1
-
1
-
1
-
-
2
-
-
-
1
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
1
-
-
1
-
-
-
2
-
-
-
-
-
1
-
-
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
682836
Li
Roles of a Trypanosoma brucei ...
Trypanosoma brucei
RNA
12
2171-2186
2006
-
1
1
-
-
-
1
-
2
-
3
-
-
1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
2
-
3
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
666249
Mukherjee
Analysis of RNA exonucleolytic ...
Homo sapiens
Methods Mol. Biol.
257
193-211
2004
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
666438
West
Human 5' --> 3' exonuclease Xr ...
Homo sapiens
Nature
432
522-525
2004
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134156
Mitkova
Emergence of the active site o ...
Bos taurus
Int. J. Biochem. Cell Biol.
31
1399-1407
1999
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134157
Mitkova
Oligomeric protein structure o ...
Bos taurus
Arch. Biochem. Biophys.
351
236-242
1998
-
-
-
-
-
-
-
-
-
-
3
1
-
2
-
1
1
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
1
-
-
1
1
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134134
Dolapchiev
Simple purification and some p ...
Bos taurus
Prep. Biochem.
12
121-136
1982
-
1
-
-
-
-
1
-
-
-
1
-
-
2
-
-
1
-
-
2
-
1
2
-
-
-
1
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
2
-
1
2
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
134138
Zielinski
Further studies on the substra ...
Bos taurus
Nucleic Acids Res.
9
235-237
1981
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
1
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134139
Hawley
Preparation, properties, and u ...
Bos taurus
Anal. Biochem.
117
18-23
1981
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134155
Bakalova-Ivanova
-
Three step procedure for purif ...
Sus scrofa
Dokl. Bolg. Akad. Nauk
31
921-924
1978
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134140
Silverman
A rapid method for preparation ...
Bos taurus
Nucleic Acids Res.
4
3511-3517
1977
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134141
Flanagan
Purification of phosphodiester ...
Cavia porcellus, Rattus norvegicus
Biochem. J.
155
607-613
1976
-
-
-
-
-
-
6
2
1
-
-
-
-
4
-
-
2
-
-
4
2
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
2
1
-
-
-
-
-
-
2
-
4
2
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
134142
Margison
Role of apurinic sites in the ...
Sus scrofa
Biochem. J.
151
249-256
1975
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134158
Kurosawa
Mechanism of DNA chain groth. ...
Sus scrofa
J. Mol. Biol.
96
653-664
1975
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134137
Philippsen
Preparation of transfer RNA fr ...
Sus scrofa
Methods Enzymol.
29E
473-477
1974
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134143
Flanagan
Phosphodiesterase II in epithe ...
Cavia porcellus, Rattus norvegicus
Biochem. J.
142
545-553
1974
-
-
-
-
-
-
-
-
1
-
2
-
-
4
-
-
-
-
-
6
-
-
2
-
-
-
2
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
6
-
-
2
-
-
-
2
-
2
2
-
-
-
-
-
-
-
-
134144
Holy
-
Substrate specifity of spleen ...
Bos taurus
Collect. Czech. Chem. Commun.
39
310-332
1974
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134145
Philippsen
Partial degradation of transfe ...
Sus scrofa
Biochim. Biophys. Acta
277
523-538
1972
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134146
Sicard
Hog spleen-phosphohydrolases-h ...
Sus scrofa
Enzymologia
43
227-244
1972
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
134135
Bernardi
-
Spleen acid exonuclease ...
Bos taurus
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
4
329-336
1971
2
-
-
-
-
-
10
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
3
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
1
-
1
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
134147
Bernardi
Action of spleen exonuclease o ...
Bos taurus
J. Biol. Chem.
244
1468-1476
1969
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
134153
Erecinska
Intracellular localization of ...
Rattus norvegicus
Eur. J. Biochem.
11
465-471
1969
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134148
Gunther
Action of spleen phosphodieste ...
Bos taurus
Arch. Biochem. Biophys.
125
480-487
1968
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
134149
Bernardi
Studies on acid hydrolases. IV ...
Sus scrofa
Biochim. Biophys. Acta
155
360-370
1968
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
1
-
-
11
-
1
-
1
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
11
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
134152
Van Dyck
-
Intracellular distribution of ...
Rattus norvegicus
Eur. J. Biochem.
7
15-20
1968
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
134136
Bernardi
-
Spleen exonuclease (1) ...
Sus scrofa
Procedures in Nucleic Acid Research (Cantoni, G. L. , Davies, D. R. , eds. )
144-153
1966
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
134150
Khorana
-
Phosphodiesterases ...
Bos taurus
The Enzymes, 2nd Ed (Boyer, P. D. , Lardy, H. , Myrbck, K. , eds. )
5
79-94
1961
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
134151
Razzell
Studies on polynucleotides ...
Bos taurus
J. Biol. Chem.
236
1144-1149
1961
-
1
-
-
-
-
2
1
-
-
-
-
-
1
-
-
1
1
-
1
5
-
4
-
-
-
-
-
1
-
-
-
1
-
-
-
1
-
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
1
-
1
5
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
134154
Heppel
-
Mechanism of enzymic hydrolysi ...
Bos taurus
Fed. Proc.
12
217
1953
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-