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BRENDA support

Literature summary for 3.1.16.1 extracted from

  • Bernardi, A.; Bernardi, G.
    Spleen acid exonuclease (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 4, 329-336.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
EDTA with RNA core as substrate Bos taurus
sulfhydryl reagents with RNA core as substrate Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
acetylation complete inhibition by acetylation of the 2'-OH of tRNA Bos taurus
arsenite weak inhibitor with RNA core as substrate Bos taurus
Cu2+
-
Bos taurus
Deoxyribonucleoside 3'-phosphates
-
Bos taurus
F- weak inhibitor with RNA core as substrate Bos taurus
glycosylation no activity with glycosylated oligonucleotides obtained from T4 phage DNA Bos taurus
Hg2+ with RNA core as substrate Bos taurus
Mg2+ with RNA core as substrate Bos taurus
Mn2+ with RNA core as substrate Bos taurus
Zn2+ with RNA core as substrate Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
spleen
-
Bos taurus
-

Storage Stability

Storage Stability Organism
-15°C, in 50% glycerol Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate
-
Bos taurus 4-nitrophenol + phosphate
-
?
bis-(4-nitrophenyl)phosphate + H2O
-
Bos taurus 4-nitrophenyl phosphate + 4-nitrophenol
-
?
DNA + H2O denatured only, no native or double stranded Bos taurus deoxyribonucleoside 3'-phosphates
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
substrate bis-(p-nitrophenyl) phosphate in 0.25 M succinate buffer Bos taurus