Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic tree | Macrovipera lebetina |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Macrovipera lebetina | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
reduced enzyme | Macrovipera lebetina |
93860 | - |
sequence calculation | Macrovipera lebetina |
120000 | - |
non-reduced enzyme | Macrovipera lebetina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Macrovipera lebetina | W8E7D1 | i.e. Macrovipera lebetina | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme is synthesized as a 828-amino acid single-chain protein but subsequently cleaved to form a two-chain protein held together with disulfide bonds | Macrovipera lebetina |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme from venom 3.2fold by gel filtration, and two different steps of anion exchange chromatography | Macrovipera lebetina |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Macrovipera lebetina | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl thymidine-5'-phosphate + H2O | best substrate | Macrovipera lebetina | 4-nitrophenol + thymidine-5'-phosphate | - |
? | |
ADP + H2O | - |
Macrovipera lebetina | AMP + phosphate | - |
? | |
bis(p-nitrophenyl)phosphate + H2O | good substrate | Macrovipera lebetina | ? | - |
? | |
additional information | the enzyme hydrolyses ADP but not ATP and 5'-AMP, the enzyme inhibits aggregation of platelets induced by ADP or collagen | Macrovipera lebetina | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme is synthesized as a 828-amino acid single-chain protein but subsequently cleaved to form a two-chain protein held together with disulfide bonds, nevertheless the enzyme behaves like a heterodimeric protein with 60000 kDa subunits, molecular modelling. The enzyme contains four domains including two somatomedin-B domains, a PDE/nucleotide pyrophosphatase domain, and a nonspecific endonuclease domain | Macrovipera lebetina |
Synonyms | Comment | Organism |
---|---|---|
VLPDE | - |
Macrovipera lebetina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Macrovipera lebetina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | - |
Macrovipera lebetina |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Macrovipera lebetina | sequence calculation | - |
6.87 |
General Information | Comment | Organism |
---|---|---|
additional information | enzyme secondary structure molecular modelling | Macrovipera lebetina |