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Literature summary for 3.1.15.1 extracted from
- An overview on nucleases (DNase, RNase, and phosphodiesterase) in snake venoms (2010), Biochemistry (Moscow), 75, 1-6.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of purified native enzyme | Trimeresurus stejnegeri | |
| 0.091 | - |
ADP | pH 7.4, 37°C | Trimeresurus stejnegeri |  |
| 0.24 | - |
nicotinamide adenine dinucleotide | pH 7.4, 37°C | Trimeresurus stejnegeri | |
| 0.29 | - |
nicotinamide guanine dinucleotide | pH 7.4, 37°C | Trimeresurus stejnegeri | |
| 0.36 | - |
ATP | pH 7.4, 37°C | Trimeresurus stejnegeri | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Trimeresurus stejnegeri | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | essential for phosphodiesterase activity of the enzyme | Trimeresurus stejnegeri | |
| Zn2+ | essential for phosphodiesterase activity of the enzyme | Trimeresurus stejnegeri | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Trimeresurus stejnegeri | the enzyme also exhibits DNase activity | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trimeresurus stejnegeri | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the venom phosphodiesterase TS-PDE is a glycoprotein which contains 2.48% carbohydrate | Trimeresurus stejnegeri |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from venom by ion exchange chromatography and gel filtration to homogeneity | Trimeresurus stejnegeri |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | commercial preparation | Trimeresurus stejnegeri | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.3 | - |
purified native enzyme, substrate nicotinamide guanine dinucleotide, pH 7.4, 37°C | Trimeresurus stejnegeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + H2O | - |
Trimeresurus stejnegeri | ? | - |
? | |
| ATP + H2O | - |
Trimeresurus stejnegeri | ? | - |
? | |
| additional information | the enzyme also exhibits DNase activity | Trimeresurus stejnegeri | ? | - |
? | |
| additional information | the purified enzyme TS-PDE exhibits exonuclease activity with a broad phosphodiesterase substrate range with the descending order of specificity: nicotinamide guanine dinucleotide, ATP, nicotinamide adenine dinucleotide, ADP | Trimeresurus stejnegeri | ? | - |
? | |
| nicotinamide adenine dinucleotide + H2O | - |
Trimeresurus stejnegeri | ? | - |
? | |
| nicotinamide guanine dinucleotide + H2O | - |
Trimeresurus stejnegeri | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TS-PDE | - |
Trimeresurus stejnegeri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Trimeresurus stejnegeri |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
ADP | pH 7.4, 37°C | Trimeresurus stejnegeri | |
| 0.82 | - |
nicotinamide adenine dinucleotide | pH 7.4, 37°C | Trimeresurus stejnegeri | |
| 2.68 | - |
ATP | pH 7.4, 37°C | Trimeresurus stejnegeri | |
| 3.65 | - |
nicotinamide guanine dinucleotide | pH 7.4, 37°C | Trimeresurus stejnegeri | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Trimeresurus stejnegeri |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the phosphodiesterase TS-PDE strongly inhibits ADP-induced platelet aggregation in human plateletrich plasma by hydrolyzing ADP | Trimeresurus stejnegeri |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.6 | - |
ADP | pH 7.4, 37°C | Trimeresurus stejnegeri | |
| 3.5 | - |
nicotinamide adenine dinucleotide | pH 7.4, 37°C | Trimeresurus stejnegeri | |
| 7.4 | - |
ATP | pH 7.4, 37°C | Trimeresurus stejnegeri | |
| 12.6 | - |
nicotinamide guanine dinucleotide | pH 7.4, 37°C | Trimeresurus stejnegeri | |
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