Literature summary for 3.1.15.1 extracted from

Valerio, A.A.; Corradini, A.C.; Panunto, P.C.; Mello, S.M.; Hyslop, S.
Purification and characterization of a phosphodiesterase from Bothrops alternatus snake venom (2002), J. Protein Chem., 21, 495-503.

Search for more literature for 3.1.15.1

General Stability

General Stability	Organism
repeated freeze-thaw cycles lead to loss of enzyme activity	Bothrops alternatus

Inhibitors

Inhibitors	Comment	Organism	Structure
2-mercaptoethanol	10 mM, 20% of inhibition	Bothrops alternatus
CuSO4	10 mM, 90% of inhibition	Bothrops alternatus
EDTA	10 mM, 90% of inhibition	Bothrops alternatus
MgSO4	100 mM, 40% of inhibition	Bothrops alternatus
MnCl2	10 mM, 50% of inhibition	Bothrops alternatus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.69	-	bis(p-nitrophenyl)-phosphate	37°C, pH 8.9	Bothrops alternatus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	10 mM, 170% of activity	Bothrops alternatus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
105000	-	PAGE	Bothrops alternatus

Organism

Organism	UniProt	Comment	Textmining
Bothrops alternatus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Bothrops alternatus

Storage Stability

Storage Stability	Organism
25°C, 0.1 M NaOH-glycine, pH 8.9, 72 h, stable	Bothrops alternatus
37°C, 0.1 M NaOH-glycine, pH 8.9, 72 h, stable	Bothrops alternatus
4°C, 0.1 M NaOH-glycine, pH 8.9, 72 h, stable	Bothrops alternatus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
bis(p-nitrophenyl)-phosphate + H2O	-	Bothrops alternatus	?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 105000, SDS-PAGE	Bothrops alternatus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	-	Bothrops alternatus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
70	-	1 min, complete loss of activity	Bothrops alternatus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	9.5	-	Bothrops alternatus

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Release 2023.1 (January 2023)