Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
initiation factor a/eIF2(gamma) | binding of translation initiation factor a/eIF2(gamma) to the 5'-end of mRNA counteracts the 5'-to-3' exoribonucleolytic activity in vitro. The factor binds by virtue of the gamma-subunit to the 5'-end of tri-phosphorylated mRNAs and thereby increases the stability of the 5'-part of mRNAs | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA + H2O | Saccharolobus solfataricus | the translation initiation factor a/eIF2(g) binds to the 5'-tri-phosphate terminus of mRNA to protect from 5'-to-3' directional decay and counteract the activity of Sso-RNase J | 3'-phosphomononucleotides | - |
? | |
RNA + H2O | Saccharolobus solfataricus P2 | the translation initiation factor a/eIF2(g) binds to the 5'-tri-phosphate terminus of mRNA to protect from 5'-to-3' directional decay and counteract the activity of Sso-RNase J | 3'-phosphomononucleotides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q980D0 | - |
- |
Saccharolobus solfataricus P2 | Q980D0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(A)40 + 38 H2O | (A)40 is a 40-nt-long synthetic RNA harboring a single radioactively labeled A nucleotide at the 5'-end. When compared with the triphosphorylated RNA, SSSO-RNase J displayes a twofold higher velocity in degrading mono-phosphorylated RNA and RNA bearing a 5'-hydroxyl group | Saccharolobus solfataricus | adenosine + 39 AMP | - |
? | |
(A)40 + 38 H2O | (A)40 is a 40-nt-long synthetic RNA harboring a single radioactively labeled A nucleotide at the 5'-end. When compared with the triphosphorylated RNA, SSSO-RNase J displayes a twofold higher velocity in degrading mono-phosphorylated RNA and RNA bearing a 5'-hydroxyl group | Saccharolobus solfataricus P2 | adenosine + 39 AMP | - |
? | |
5'-pA(A)39 + H2O | 5'-pA(A)39 is a 40-nt-long synthetic RNA harboring a 5'-monophosphate and a single radioactively labeled A nucleotide at the 5'-end. When compared with the triphosphorylated RNA, the enzyme displayes a twofold higher velocity in degrading mono-phosphorylated RNA and RNA bearing a 5'-hydroxyl group | Saccharolobus solfataricus | 40 AMP | - |
? | |
5'-pA(A)39 + H2O | 5'-pA(A)39 is a 40-nt-long synthetic RNA harboring a 5'-monophosphate and a single radioactively labeled A nucleotide at the 5'-end. When compared with the triphosphorylated RNA, the enzyme displayes a twofold higher velocity in degrading mono-phosphorylated RNA and RNA bearing a 5'-hydroxyl group | Saccharolobus solfataricus P2 | 40 AMP | - |
? | |
5'-pppA(A)39 + H2O | 5'-pppA(A)39 is a 40-nt-long synthetic RNA harboring a 5'-triphosphate and a single radioactively labeled A nucleotide at the 5'-end. When compared with the triphosphorylated RNA, the enzyme displayes a twofold higher velocity in degrading mono-phosphorylated RNA and RNA bearing a 5'-hydroxyl group | Saccharolobus solfataricus | ATP + 39 AMP | - |
? | |
5'-pppA(A)39 + H2O | 5'-pppA(A)39 is a 40-nt-long synthetic RNA harboring a 5'-triphosphate and a single radioactively labeled A nucleotide at the 5'-end. When compared with the triphosphorylated RNA, the enzyme displayes a twofold higher velocity in degrading mono-phosphorylated RNA and RNA bearing a 5'-hydroxyl group | Saccharolobus solfataricus P2 | ATP + 39 AMP | - |
? | |
additional information | using synthetic RNAs as well as 5'-end-labeled natural mRNAs from Sulfolobus solfataricus, no hints for an endonucleolytic activity are obtained | Saccharolobus solfataricus | ? | - |
? | |
additional information | using synthetic RNAs as well as 5'-end-labeled natural mRNAs from Sulfolobus solfataricus, no hints for an endonucleolytic activity are obtained | Saccharolobus solfataricus P2 | ? | - |
? | |
RNA + H2O | the translation initiation factor a/eIF2(g) binds to the 5'-tri-phosphate terminus of mRNA to protect from 5'-to-3' directional decay and counteract the activity of Sso-RNase J | Saccharolobus solfataricus | 3'-phosphomononucleotides | - |
? | |
RNA + H2O | the translation initiation factor a/eIF2(g) binds to the 5'-tri-phosphate terminus of mRNA to protect from 5'-to-3' directional decay and counteract the activity of Sso-RNase J | Saccharolobus solfataricus P2 | 3'-phosphomononucleotides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5'-to-3' exoribonuclease | - |
Saccharolobus solfataricus |
Sso-RNase J | misleading | Saccharolobus solfataricus |
SSO0386 | locus name | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Saccharolobus solfataricus |