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Literature summary for 3.1.13.B1 extracted from

  • Märtens, B.; Amman, F.; Manoharadas, S.; Zeichen, L.; Orell, A.; Albers, S.V.; Hofacker, I.; Bläsi, U.
    Alterations of the transcriptome of Sulfolobus acidocaldarius by exoribonuclease aCPSF2 (2013), PLoS One, 8, e76569.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.13.B1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia col Sulfolobus acidocaldarius

Protein Variants

Protein Variants Comment Organism
additional information to address the biological significance of Saci-aCPSF2, a deletion mutant is constructed, and the influence of SaciaCPSF2 on the transcriptome profile is assessed employing high throughput RNA sequencing Sulfolobus acidocaldarius

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activity is dependent on Mg2+ Sulfolobus acidocaldarius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
RNA + H2O Sulfolobus acidocaldarius the enzyme plays a major role in mRNA turnover 3'-phosphomononucleotides
-
 ?
RNA + H2O Sulfolobus acidocaldarius DSM 639 the enzyme plays a major role in mRNA turnover 3'-phosphomononucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius Q4J6E1
-
-
Sulfolobus acidocaldarius DSM 639 Q4J6E1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-pppA(A)41 + H2O 5'-pppA(A)41 is a 42-nt-long synthetic RNA harboring a 5'-tri-phosphate and a single radioactively labeled A nucleotide at the 5'-end. Decay of 5'-PPP-40A1 RNA results in either a single nucleotide or short oligoribonucleotides, indicating an exoribonucleolytic activity. Degradation with 5' to 3' directionality in vitro Sulfolobus acidocaldarius ATP + 41 AMP
-
 ?
5'-pppA(A)41 + H2O 5'-pppA(A)41 is a 42-nt-long synthetic RNA harboring a 5'-tri-phosphate and a single radioactively labeled A nucleotide at the 5'-end. Decay of 5'-PPP-40A1 RNA results in either a single nucleotide or short oligoribonucleotides, indicating an exoribonucleolytic activity. Degradation with 5' to 3' directionality in vitro Sulfolobus acidocaldarius DSM 639 ATP + 41 AMP
-
 ?
RNA + H2O the enzyme plays a major role in mRNA turnover Sulfolobus acidocaldarius 3'-phosphomononucleotides
-
 ?
RNA + H2O the enzyme plays a major role in mRNA turnover Sulfolobus acidocaldarius DSM 639 3'-phosphomononucleotides
-
 ?

Synonyms

Synonyms Comment Organism
Saci-aCPSF2
-
 Sulfolobus acidocaldarius
Saci2362 locus name Sulfolobus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 assay at Sulfolobus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Sulfolobus acidocaldarius

General Information

General Information Comment Organism
physiological function the enzyme plays a major role in mRNA turnover Sulfolobus acidocaldarius