Cloned (Comment) | Organism |
---|---|
- |
Xenopus laevis |
expression in Escherichia coli BL21 (DE3) | Xenopus laevis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Xenopus laevis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | nocturnin is expressed in the cytoplasm of rod and cone photoreceptor cells | Xenopus laevis | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | nocturnin nuclease activity is magnesium dependent | Xenopus laevis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xenopus laevis | P79942 | - |
- |
Xenopus laevis | Q90ZA1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Xenopus laevis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
photoreceptor cell | expression of nocturnin mRNA is confined to the clock-containing photoreceptor cell layer within the retina | Xenopus laevis | - |
retina | - |
Xenopus laevis | - |
retina | constitutively present in most retinal cell | Xenopus laevis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
mRNA-poly(A) + H2O | nocturnin removes the poly(A) tail from a synthetic RNA substrate in a process known as deadenylation. It specifically degrades the 3' poly(A) tail | Xenopus laevis | ? | - |
? | |
mRNA-poly(A) + H2O | - |
Xenopus laevis | 5'-AMP + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PARN | - |
Xenopus laevis |
Organism | Comment | Expression |
---|---|---|
Xenopus laevis | nocturnin is rhythmically expressed in the cytoplasm of the retinal retina photoreceptor cells, with peak mRNA levels in the early night | up |
General Information | Comment | Organism |
---|---|---|
physiological function | while nocturnin is rhythmically expressed in the cytoplasm of the retinal photoreceptor cells, the only other described vertebrate deadenylase, PARN, is constitutively present in most retinal cells, including the photoreceptors. The distinct spatial and temporal expression of nocturnin and PARN suggests that there may be specific mRNA targets of each deadenylase | Xenopus laevis |
physiological function | while nocturnin is rhythmically expressed in the cytoplasm of the retinal photoreceptor cells, the only other described vertebrate deadenylase, PARN, is constitutively present in most retinal cells, including the photoreceptors. The distinct spatial and temporal expression of nocturnin and PARN suggests that there may be specific mRNA targets of each deadenylase. Since deadenylation regulates mRNA decay and/or translational silencing, it is proposed that nocturnin deadenylates clock-related transcripts in a novel mechanism for posttranscriptional regulation in the circadian clock or its outputs | Xenopus laevis |