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Literature summary for 3.1.13.4 extracted from

  • Montellese, C.; Montel-Lehry, N.; Henras, A.K.; Kutay, U.; Gleizes, P.E.; ODonohue, M.F.
    Poly(A)-specific ribonuclease is a nuclear ribosome biogenesis factor involved in human 18S rRNA maturation (2017), Nucleic Acids Res., 45, 6822-6836 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.13.4

Cloned(Commentary)

Cloned (Comment) Organism
gene PARN, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta Homo sapiens

Protein Variants

Protein Variants Comment Organism
H377A site-directed mutagenesis, catalytic inactive mutant Homo sapiens
additional information enzyme knockout by siRNA in HeLa cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information PARN co-purifies with 40S subunit precursors Homo sapiens
-
-
nucleolus nucleolar steady-state localization Homo sapiens 5730
-
nucleus PARN occurs in the nucleus, upstream of the final endonucleolytic cleavage by the endonuclease NOB1 in the cytoplasm Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O95453
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta by nickel affinity tandem chromatography and anion exchange chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
18S-E pre-rRNA + H2O different 18S-E pre-rRNAs Homo sapiens ?
-
 ?
additional information enzyme PARN performs exonucleolytic trimming of 18S-E pre-rRNA, and PARN can process the corresponding ITS1 RNA fragment in vitro Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
PARN
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

General Information

General Information Comment Organism
malfunction knocking down ribosome biogenesis factors (RBFs) involved in the large subunit pathway (LSG1 and eIF6) has no effect on PARN localization. Co-depletion of PARN and NOB1 yields a much stronger accumulation of 18S-EFL species than the sole knockdown of PARN. This suggests that NOB1 can also cleave untrimmed 18S-E pre-rRNAs, albeit less efficiently. In accordance with this assumption, overexpression of NOB1 in PARN-depleted cells leads to a decreased amount of 18S-EFL pre-rRNA Homo sapiens
metabolism the enzyme plays a role in mRNA catabolism and in exonucleolytic trimming of 18S-E pre-rRNA Homo sapiens
physiological function poly(A)-specific ribonuclease is a nuclear ribosome biogenesis factor involved in human 18S rRNA maturation. The enzyme supports the processing of different types of non-coding RNAs including telomerase RNA. PARN is part of the enzymatic machinery that matures the human 18S ribosomal RNA (rRNA). PARN is required for 40S ribosomal subunit production. Function of PARN in exonucleolytic trimming of 18S-E pre-rRNA. A number of nucleolar 40S-specific ribosome biogenesis factors (RBFs), including ENP1, DIM2, RRP12, LTV1 and TSR1, accompany pre-40S particles from the nucleolus via the nucleoplasm to the cytosol. Like shuttling nucleolar RBFs, PARN redistributes from the nucleolus to the nucleoplasm upon inhibition of pre-40S particle export by leptomycin B. PARN is coupled to a poly(A) polymerase for processing of the 18S-E pre-rRNA. PARN is primarily involved in pre-rRNA processing, not in quality control Homo sapiens